Home Endocrinology & Hormones ACE inhibitor DX600 For research use only.

DX600

DX600 is a specific inhibitor of angiotensin-converting enzyme 2 (ACE2) that does not cross-react with ACE.

DX600 Chemical Structure

DX600 Chemical Structure

CAS: 478188-26-0

Selleck's DX600 has been cited by 2 publications

Purity & Quality Control

Batch: S966601 Water] 100 mg/mL] false] ] ] false] ] ] false Purity: 99.19%
99.19

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Biological Activity

Description DX600 is a specific inhibitor of angiotensin-converting enzyme 2 (ACE2) that does not cross-react with ACE.
Targets
ACE2 [1]
In vitro
In vitro

DX600 is an ACE2 specific inhibitor that does not cross-react with ACE. Three human cell lines (Calu-3, Caco-2, Huh-7) are evaluated for the cell surface expression of ACE2 by flow cytometry and Western blot. Subsequently, whole cell lysates, cell culture supernatant, and live cells are evaluated in the assay. Calu-3 has elevated levels of ACE2 compared to Caco-2 or Huh-7. Calu-3 also demonstrates elevated ACE2 enzymatic activity in all three sources and can be inhibited by DX600.[1]
Cell Research Cell lines Three human cell lines (Calu-3, Caco-2, Huh-7)
Concentrations 10 μM
Incubation Time 1 h
Method

To measure NAb activity against endogenous ACE2, the recombinant ACE2 is replaced by cellular ACE2. 100 μL of cell lysate at 4 mg/mL or same volume of cell culture supernatant is pre-incubated with either PCs at a final concentration of 10 μg/mL, equal volume control serum, DX600, ACE2 specific inhibitor at final concentration of 10 μM, or equal volume of control buffer (PBS) at room temperature for 1 h.
In Vivo
In vivo

Mouse ACE2-driven Ang-(1-7) formation from Ang II is blocked by MLN-4760 but not by either linear or cyclic DX600. DX600 only blocks human ACE2 activity.[2]

Chemical lnformation & Solubility

Molecular Weight 3074.32 Formula

C141H185N35O40S2
CAS No. 478188-26-0 SDF --
Smiles CC(C)CC1NC(=O)C2CCCN2C(=O)C(CO)NC(=O)C(CSSCC(NC(=O)C(CCCCN)NC(=O)C(CC3=C[NH]C4=C3C=CC=C4)NC(=O)C(CC5=C[NH]C6=C5C=CC=C6)NC(=O)C7CCCN7C(=O)C(CC8=CC=C(O)C=C8)NC(=O)C(CC9=CC=C(O)C=C9)NC(=O)C(CCCNC(N)=N)NC1=O)C(=O)NC(C(C)O)C(=O)NC(CC%10=CC=C(O)C=C%10)C(=O)N%11CCCC%11C(=O)NC(CC(O)=O)C(=O)N%12CCCC%12C(=O)NC(CCC(O)=O)C(=O)NCC(=O)NCC(=O)NCC(N)=O)NC(=O)C(CC%13=CN=C[NH]%13)NC(=O)C(CO)NC(=O)C(CC%14=CC=C(O)C=C%14)NC(=O)C(CC(O)=O)NC(=O)CNC(C)=O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

Water : 100 mg/mL


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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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