Home Proteases 3C-Like Protease inhibitor GC376 For research use only.

GC376

GC376 is a 3CLpro (3C-like protease) inhibitor with IC50 of ~ 1.11 μM for the PEDV 3CLpro. GC376 is active against the 3CLpro of multiple coronaviruses, including SARS-CoV.

GC376 Chemical Structure

GC376 Chemical Structure

CAS: 1416992-39-6

Selleck's GC376 has been cited by 21 publications

Purity & Quality Control

Batch: Purity: >97%
97

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Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HG23 Antiviral assay Antiviral activity against Norovirus infected in human HG23 cells, ED50 = 0.3 μM. 23218713
Click to View More Cell Line Experimental Data

Biological Activity

Description GC376 is a 3CLpro (3C-like protease) inhibitor with IC50 of ~ 1.11 μM for the PEDV 3CLpro. GC376 is active against the 3CLpro of multiple coronaviruses, including SARS-CoV.
Targets
PEDV 3CLpro [1]
(Cell-free assay)
1.11 μM
In vitro
In vitro

Molnupiravir and GC376 show a synergistic activity on SARS-CoV-2 at 48 h, and an additive activity on SARS-CoV-2 at 72 h.[2]
Cell Research Cell lines Vero E6 cells
Concentrations 0.21-16.7 μM
Incubation Time 48 and 72 h
Method

Vero E6 cells (3000 cells/well) were seeded in 96-well clear flat-bottom plates and incubated at 37°C with 5% CO2 for 24 h. After incubation, cells were infected using a multiplicity of infection (MOI) of 0.1. SARS-CoV-2 was allowed to adsorb for one hour at 37°C. Subsequently, virus inoculum was removed, and cells were overlaid with media containing 3-fold serial dilutions of molnupiravir (0.62-50 μM), nirmatrelvir (0.62-50 μM) and GC376 (0.21-16.7 μM). Negative controls (compounds alone), infected positive controls (SARS-CoV-2 alone) and mock-infected cells were included in each plate. Plates were incubated at 37°C with 5% CO2 for 48 and 72 h and then, cell viability was measured using an MTT reduction assay.

(Data sourced from selleck products)
In Vivo
In vivo

GC376, a dipeptidyl bisulfite adduct salt, is a 3CLpro (3C-like protease) inhibitor that exerts strong inhibitory effects on picornaviruses and coronaviruses, including SARS-CoV.
Animal Research Animal Models Female BALB/c mice
Dosages 111 or 55.5 mg/kg
Administration i.m.

Chemical lnformation & Solubility

Molecular Weight 507.53 Formula

C21H30N3NaO8S
CAS No. 1416992-39-6 SDF --
Smiles CC(C)CC(C(=O)NC(CC1CCNC1=O)C(O)S(=O)(=O)[O-])NC(=O)OCC2=CC=CC=C2.[Na+]
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (197.03 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 100 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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