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Guanidine HCl

Synonyms: Aminoformamidine HCl

Guanidine HCl (Aminoformamidine), the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant.

Guanidine HCl Chemical Structure

Guanidine HCl Chemical Structure

CAS: 50-01-1

Selleck's Guanidine HCl has been cited by 1 publication

Purity & Quality Control

Batch: S407001 DMSO] 19 mg/mL] false] Water] 19 mg/mL] false] Ethanol] 6 mg/mL] false Purity: 100.00%
100.00

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Biological Activity

Description Guanidine HCl (Aminoformamidine), the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant.
In vitro
In vitro Guanidine HCl is the most popular protein denaturant. Analysis of unfolding transitions by Guanidine HCl provides several important parameters regarding the mechanism of conformational stability of proteins. [1] Guanidine HCl at low concentrations refolds acid-unfolds apomyoglobin and cytochrome c, stabilizing the molten globule state. Guanidine HCl (> 1 M) causes co-operative unfolding of the molten globule state. [2] Guanidine HCl is 2.8 times more effective than urea in unfolding ribonuclease but only 1.7 times more effective for lysozyme. △GH2Oapp. values of Guanidine HCl are 9.7 Cal/mole for ribonuclease at pH 6.6, 6.1 for lysozyme at pH 2.9, 8.3 for α-chymotrypsin at pH 4.3, and 11.7 for β-lactoglobulin at pH 3.2. [3] Guanidine HCl at millimolar concentrations, is able to causes efficient loss of the normally stable [PSI+] element from yeast cells. 5 mM Guanidine HCl in growth media cures [PSI+] and other prions of yeast. [4] 5 mM Guanidine HCl significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold, respectively. Guanidine HCl also reduces the ability of Hsp104 to restore activity of thermally denatured luciferase. [5]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03658902 Completed Botulism; Poisoning Centre Hospitalier le Mans January 1 2000 --

Chemical lnformation & Solubility

Molecular Weight 95.53 Formula

CH5N3.HCl
CAS No. 50-01-1 SDF Download Guanidine HCl SDF
Smiles C(=N)(N)N.Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 19 mg/mL ( (198.89 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 19 mg/mL

Ethanol : 6 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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