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JC-1

Name: JC-1
Brand: Selleck Chemicals
SKU: S9784
Rating: 5 (2 reviews)

Synonyms: CBIC2, NK 1420

Catalog No.S9784 Purity: 99.34%

JC-1 (CBIC2, NK 1420), a fluorescent lipophilic carbocyanine dye, is a mitochondrial potential (ΔΨ(m)) marker. JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers.

JC-1 Chemical Structure

CAS: 3520-43-2

Selleck's JC-1 has been cited by 2 publications

Purity & Quality Control

Batch: S978401 DMSO] 33 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.34%

99.34

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Biological Activity

Description	JC-1 (CBIC2, NK 1420), a fluorescent lipophilic carbocyanine dye, is a mitochondrial potential (ΔΨ(m)) marker. JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers.

In vitro
In vitro	1. Take the 6-well plate as an example for cell planking, and the density is 5×10^5/mL. Incubate overnight in 5% CO2 incubator at 37℃. Note: it is suggested that the cell density during apoptosis induction should not exceed 1×10^6/ml, which can also be cultured to the appropriate density according to your own cell type. 2. Take 0.5 mL suspension into sterile centrifuge tube; 400 g centrifugation for 3-5 min; Discard the supernatant. 3. The cells were resuspended with 1mljc-1 working solution and incubated in 5% CO2 incubator at 37℃for 15-30 min. 4. Centrifugation at room temperature for 5 min at 400 g; Suck of the supernatant. 5. The cells were resuspended with 2 mL cell culture medium or buffer, and then centrifuged at room temperature for 5 min at 400 g; Discard the supernatant and repeat twice. 6. Resuspend the cells with 1mL of fresh culture medium or buffer, and immediately conduct subsequent flow cytometry or fluorescence microscope observation. 7. Data analysis (flow cytometry) : mitochondria of healthy cells containing red JC-1 aggregates were detected by FL2 channel; Apoptotic or unhealthy cells containing green JC-1 monomer were detected by FL1 (FITC) channel. 8. If used for enzyme labeling instrument, use 300 μL buffer resuspended cells; Then 100 per hole μ Transfer the stained cells to a light tight 96 well plate with the amount of L, and then conduct fluorescent enzyme label plate analysis.
Cell Research	Cell lines	hippocampal neurons and glial cells
	Concentrations	1 μg/ml
	Incubation Time	15 min
	Method	JC-1 is dissolved in dimethylsulfoxide (DMSO) as a 2 mg/ml stock. JC-1 is excited at 490 nm and an optical image splitter device is attached to the microscope to separate spectrally the green and red components of JC-1 fluorescence.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT03161561	Completed	COPD	Sheffield Teaching Hospitals NHS Foundation Trust	November 16 2011	Not Applicable

References

Chemical lnformation & Solubility

Molecular Weight	560.77	Formula	C₂₅H₂₇Cl₅N₄
CAS No.	3520-43-2	SDF	--
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 33 mg/mL ( (58.84 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molecular Weight Calculator

In vivo
Batch:

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In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

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% DMSO + % + % Tween 80 + % ddH₂O

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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