Home Proteases Cysteine Protease inhibitor - COVID-19 inhibitor Loxistatin Acid (E-64C) For research use only.

Loxistatin Acid (E-64C)

Synonyms: NSC 694279, EP 475

Loxistatin Acid (E-64C, NSC 694279, EP 475), a derivative of E-64, is an irreversible and membrane-permeant cysteine protease inhibitor. The cysteine protease cathepsin L is required for SARS-CoV-2 viral entry.

Loxistatin Acid (E-64C) Chemical Structure

Loxistatin Acid (E-64C) Chemical Structure

CAS: 76684-89-4

Selleck's Loxistatin Acid (E-64C) has been cited by 3 publications

Purity & Quality Control

Batch: S739201 DMSO] 62 mg/mL] false] Ethanol] 62 mg/mL] false] Water] 2 mg/mL] false Purity: 99.82%
99.82

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Biological Activity

Description Loxistatin Acid (E-64C, NSC 694279, EP 475), a derivative of E-64, is an irreversible and membrane-permeant cysteine protease inhibitor. The cysteine protease cathepsin L is required for SARS-CoV-2 viral entry.
Targets
cysteine protease [1]
In vitro
In vitro Loxistatin Acid (E-64C), as a thiol protease inhibitor, inhibits calpain activity in intact platelets. [1]E-64C inhibits β-secretase activity in regulated secretory vesicles of neuronal chromaffin cells, and decrease Aβ production, indicating a therapeutic strategy for Alzheimer's disease. [4]
In Vivo
In vivo In pectoral muscle of dystrophic chickens, Loxistatin Acid (E-64C) normalizes the increased activities of cathepsin B and cathepsin H. [2] E-64C inhibits ischemic degradation of cerebral proteins following occlusion of the middle cerebral artery in rats. [3]
Animal Research Animal Models Male Sprague-Dawley rats.
Dosages ~400 mg/kg
Administration i.p.

Chemical lnformation & Solubility

Molecular Weight 314.38 Formula

C15H26 N2 O5
CAS No. 76684-89-4 SDF Download Loxistatin Acid (E-64C) SDF
Smiles CC(C)CCNC(=O)C(CC(C)C)NC(=O)C1C(O1)C(=O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 62 mg/mL ( (197.21 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 62 mg/mL

Water : 2 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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