Home Neuronal Signaling AChR agonist Methacholine chloride For research use only.

Methacholine chloride

Synonyms: Mecholyl chloride, Acetyl-β-methylcholine chloride

Methacholine (Mecholyl, Acetyl-β-methylcholine) is a synthetic choline ester that acts as a non-selective muscarinic receptor agonist in the parasympathetic nervous system but has little effects on the nicotinic receptors.

Methacholine chloride Chemical Structure

Methacholine chloride Chemical Structure

CAS: 62-51-1

Purity & Quality Control

Batch: S368401 DMSO] 39 mg/mL] false] Water] 39 mg/mL] false] Ethanol] 39 mg/mL] false Purity: 99.64%
99.64

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Biological Activity

Description Methacholine (Mecholyl, Acetyl-β-methylcholine) is a synthetic choline ester that acts as a non-selective muscarinic receptor agonist in the parasympathetic nervous system but has little effects on the nicotinic receptors.
Targets
muscarinic receptor [1]
In vitro
In vitro Methacholine induced modest effects on basal interleukin (IL)-8 and -6 secretion, whereas the secretion of RANTES, eotaxin, vascular endothelial growth factor-A and monocyte chemoattractant protein-1 was not affected[1].
Cell Research Cell lines Human airway smooth muscle cells
Concentrations 10 μM
Incubation Time 24 h
Method Human airway smooth muscle cells are grown to confluence and subsequently serum-deprived for 1 day in ITS-supplemented media. Cells are then treated with increasing concentrations of CSE, in the absence or the presence of methacholine (10 μM). Supernatants are then analysed for the presence of IL-8 and IL-6.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00720590 Completed Endothelial Dysfunction Indiana University|National Heart Lung and Blood Institute (NHLBI) November 2003 Not Applicable

Chemical lnformation & Solubility

Molecular Weight 195.69 Formula

C8H18NO2.Cl
CAS No. 62-51-1 SDF Download Methacholine chloride SDF
Smiles CC(C[N+](C)(C)C)OC(=O)C.[Cl-]
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 39 mg/mL ( (199.29 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 39 mg/mL

Ethanol : 39 mg/mL


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