Home Microbiology Antibiotics chemical Minocycline HCl For research use only.

Minocycline HCl

Synonyms: CL 59806

Minocycline HCl is the most lipid soluble and most active tetracycline antibiotic, binds to the 30S ribosomal subunit, preventing the binding of tRNA to the mRNA-ribosome complex and interfering with protein synthesis.

Minocycline HCl Chemical Structure

Minocycline HCl Chemical Structure

CAS: 13614-98-7

Selleck's Minocycline HCl has been cited by 34 publications

Purity & Quality Control

Batch: Purity: 99.94%
99.94

Related compound libraries

Choose Selective Antibiotics Inhibitors

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Jurkat cells Cell viability assay 10-200 µM 24 h reduces cell viability and induces DNA fragmentation and dissipation of the mitochondrial membrane potential in Jurkat cells but is harmless to human peripheral blood lymphocyte cells (hPBLCs) 29625165
hPBLCs Cell viability assay 10-200 µM 24 h reduces cell viability and induces DNA fragmentation and dissipation of the mitochondrial membrane potential in Jurkat cells but is harmless to human peripheral blood lymphocyte cells (hPBLCs) 29625165
Click to View More Cell Line Experimental Data

Biological Activity

Description Minocycline HCl is the most lipid soluble and most active tetracycline antibiotic, binds to the 30S ribosomal subunit, preventing the binding of tRNA to the mRNA-ribosome complex and interfering with protein synthesis.
In vitro
In vitro

Minocycline is a second-generation tetracycline used in humans, which effectively crosses the blood−brain barrier. It inhibits the activity of caspase-1, caspase-3, inducible form of nitric oxide synthetase (iNOS) and p38 mitogen-activated protein kinase (MAPK). After experimental ischemia, minocycline inhibits caspase-1 and inducible nitric oxide synthetase (iNOS) upregulation, and decreases infarct size. Minocycline inhibits mitochondrial permeability-transition-mediated cytochrome c release. Minocycline-mediated inhibition of cytochrome c release is demonstrated in vivo, in cells, and in isolated mitochondria. [2]
Cell Research Cell lines OVCAR-3, SKOV-3 and A2780 cells
Concentrations IC50 values of 62.0, 56.1 and 59.5 μM, respectively
Incubation Time 72 h
Method

Cells were treated with various concentrations of drug for 72 h.
Experimental Result Images Methods Biomarkers Images PMID
Western blot IL-6Rα / gp130 p-STAT3 / STAT3 / Mcl-1 / p-ERK / ERK 23593315
Immunofluorescence p-STAT3 STAT3 23593315
Growth inhibition assay Cell viability 27555377
In Vivo
In vivo

Minocycline is a semi-synthetic tetracycline derivative which is well absorbed and distributed in body tissues and is suitable for twice daily administration. Minocycline's effects are related to the inhibition of protein synthesis. Although minocycline's broader spectrum of activity, compared to other members of the group, includes activity against Neisseria meningitidis, its use as a prophylaxis is no longer recomended because of side effects (dizziness and vertigo). [1]

Minocycline is remarkable neuroprotective qualities in models of cerebral ischaemia, traumatic brain injury, and Huntington's and Parkinson's disease. The neuroprotective action of minocycline may include its inhibitory effect on 5-lipoxygenase, an inflammatory enzyme associated with brain aging. [3]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05605366 Not yet recruiting Sickle Cell Disease|Cognitive Impairment|Cognitive Decline|Cognitive Change|Cognitive Dysfunction|Cognitive Deficit|Neuroinflammatory Response University of Cincinnati June 1 2024 Phase 1
NCT05861258 Recruiting Mycobacterium Avium Complex Pulmonary Disease Radboud University Medical Center May 8 2023 Phase 2
NCT05630534 Not yet recruiting Intracerebral Hemorrhage Second Affiliated Hospital School of Medicine Zhejiang University|The Fourth Affiliated Hospital of Zhejiang University School of Medicine|The Second Affiliated Hospital of Jiaxing University January 1 2023 Phase 1
NCT05680389 Recruiting Cerebral Amyloid Angiopathy Leiden University Medical Center December 2 2020 Phase 1|Phase 2

Chemical lnformation & Solubility

Molecular Weight 493.94 Formula

C23H27N3O7.HCl
CAS No. 13614-98-7 SDF Download Minocycline HCl SDF
Smiles CN(C)C1C2CC3CC4=C(C=CC(=C4C(=C3C(=O)C2(C(=C(C1=O)C(=O)N)O)O)O)O)N(C)C.Cl
Storage (From the date of receipt)

In vitro
Batch:

Water : 6 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Minocycline HCl | Minocycline HCl ic50 | Minocycline HCl price | Minocycline HCl cost | Minocycline HCl solubility dmso | Minocycline HCl purchase | Minocycline HCl manufacturer | Minocycline HCl research buy | Minocycline HCl order | Minocycline HCl mouse | Minocycline HCl chemical structure | Minocycline HCl mw | Minocycline HCl molecular weight | Minocycline HCl datasheet | Minocycline HCl supplier | Minocycline HCl in vitro | Minocycline HCl cell line | Minocycline HCl concentration | Minocycline HCl nmr