Home DNA Damage/DNA Repair Topoisomerase inhibitor Pefloxacin Mesylate Dihydrate For research use only.

Pefloxacin Mesylate Dihydrate

Synonyms: 1589 RB,Pefloxacinium mesylate dihydrate

Pefloxacin Mesylate Dihydrate (1589 RB,Pefloxacinium mesylate dihydrate) is the third generation of fluoroquinolone class of antibacterials, which inhibits Topoisomerase II activity and DNA replication.

Pefloxacin Mesylate Dihydrate Chemical Structure

Pefloxacin Mesylate Dihydrate Chemical Structure

CAS: 149676-40-4

Purity & Quality Control

Batch: S411901 Water] 67 mg/mL] false] DMSO] 9 mg/mL] false] Ethanol] Insoluble] false Purity: 99.96%
99.96

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Biological Activity

Description Pefloxacin Mesylate Dihydrate (1589 RB,Pefloxacinium mesylate dihydrate) is the third generation of fluoroquinolone class of antibacterials, which inhibits Topoisomerase II activity and DNA replication.
Targets
Topo II [1]
In vitro
In vitro

Pefloxacin like that of other quinolones mainly targets bacterial DNA gyrase (topoisomerase II) which is an essential bacterial enzyme with a variety of activities. Pefloxacin is a strongly bactericidal in a complex manner; the bactericidal effect has been described as 'biphasic' with a single rapidly killing effect followed by a paradoxical reduced killing phase in the presence of increased concentration; in bacterial cells incubated with Pefloxacin, non-replicative DNA synthesis (DNA repair), termed SOS response, is induced and SOS response prevents replication, blocking cell division and producing filarnentation. Thus the end result of the SOS response is harmful for the bacteria with morphological and biochemical disturbances. Pefloxacin is highly active against the Enterobacteriaceae (Citrobacter, Enterobacter, Klebsiella, Morganella, Proteus); the minimal inhibitory concentrations (MICs) of Pefloxacin for all genus and species of this family of bacteria ranges usually between 0.03 and 4 or 8 mg/L; against Salmonella, Shigella and Yersinia the MIC50/MIC90 are lower: 0.12/1.0, 0.06/0.06, 0.12/0.25 mg/L, respectively. The MICs of Pefloxacin for Gram- negative aerobic bacilli, such as Pseudomonas aeruginosa, Acinetobacter spp., Alcaligenes, Pseudomonas spp. , ranges from 1 to 4 mg/L. Gram-positive cocci Staphylococci including S. aureus and coagulase-negative staphylococci even resistant to other antibiotics are susceptible or moderately susceptible to Pefloxacin, with MICs in the range 0.125-0.5 mg/L. The MICs of Pefloxacin for Legionella pneumophila are moderately low (MIC50/MIC90:1.0/1.0mg/L); for Eikenella corrodens, Vibrio cholerae, the MICs are very low, whereas Helicobacter pylori, Listeria monocytogenes and Nocardia astero'ides are resistant to Pefloxacin, with MIC50 of 8 mg/L or more. Mycobacteria are moderately or not susceptible to Pefloxacin and most anaerobes are resistant to Pefloxacin. Mycoplasma spp and Chlamydiae are poorly susceptible to Pefloxacin (MIC50/MIC90: 2/8 mg/L) whereas Rickettsia conori and Coxiella burneti are susceptible at therapeutically achievable concentrations. [1]

Chemical lnformation & Solubility

Molecular Weight 465.49 Formula

C17H20FN3O3.CH4O3S.2H2O
CAS No. 149676-40-4 SDF Download Pefloxacin Mesylate Dihydrate SDF
Smiles CCN1C=C(C(=O)C2=CC(=C(C=C21)N3CCN(CC3)C)F)C(=O)O.CS(=O)(=O)O.O.O
Storage (From the date of receipt)

In vitro
Batch:

Water : 67 mg/mL

DMSO : 9 mg/mL ( (19.33 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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