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Pilocarpine HCl

Name: Pilocarpine HCl
Brand: Selleck Chemicals
SKU: S4231
Rating: 5 (8 reviews)

Synonyms: NSC 5746 HCl

Catalog No.S4231 Purity: 99.98%

Pilocarpine HCl (NSC 5746) is a nonselective muscarinic acetylcholine receptor agonist used to produce an experimental model of epilepsy.

Pilocarpine HCl Chemical Structure

CAS: 54-71-7

Selleck's Pilocarpine HCl has been cited by 8 publications

Purity & Quality Control

Batch: Purity: 99.98%

99.98

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Biological Activity

Description

Pilocarpine HCl (NSC 5746) is a nonselective muscarinic acetylcholine receptor agonist used to produce an experimental model of epilepsy.

Targets

mAChR ^[1]

In vitro
In vitro	Pilocarpine is a parasympathomimetic alkaloid obtained from the leaves of tropical American shrubs from the genus Pilocarpus. It is a non-selective muscarinic receptor agonist in the parasympathetic nervous system, which acts therapeutically at themuscarinic acetylcholine receptor M3 due to its topical application, e.g., in glaucoma and xerostomia. Pilocarpine acts on a subtype of muscarinic receptor (M3) found on the iris sphincter muscle, causing the muscle to contract and engage in miosis. ^[1] Pilocarpine also acts on the ciliary muscle and causes it to contract. When the ciliary muscle contracts, it opens the trabecular meshwork through increased tension on the scleral spur. This action facilitates the rate that aqueous humorleaves the eye to decrease intraocular pressure. In ophthalmology, pilocarpine is also used to reduce the possibility of glare at night from lights when the patient has undergone implantation of phakic intraocular lenses; the use of pilocarpine would reduce the size of the pupils, relieving these symptoms. The most common concentration for this use is pilocarpine 1%, the weakest concentration. Pilocarpine is also used to treat dry mouth (xerostomia) which can occur, for example, as a side effect of radiation therapy for head and neck cancers. Pilocarpine stimulates the secretion of large amounts of saliva and sweat.^[2]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT05506137	Not yet recruiting	Xerostomia\|Dry Mouth	Maastricht University Medical Center\|ZonMw: The Netherlands Organisation for Health Research and Development	January 2023	Phase 3
NCT05564832	Recruiting	Near Vision	Shahid Beheshti University of Medical Sciences	August 1 2022	Early Phase 1
NCT05006898	Completed	Presbyopia	Optall Vision	June 1 2020	Phase 1
NCT04005079	Withdrawn	Open Angle Glaucoma\|Ocular Hypertension	Montefiore Medical Center	June 5 2019	Phase 3
NCT03912792	Withdrawn	XLHED	Dermelix Biotherapeutics LLC.	June 28 2019	--

References

Chemical lnformation & Solubility

Molecular Weight	244.72	Formula	C₁₁H₁₆N₂O₂.HCl
CAS No.	54-71-7	SDF	Download Pilocarpine HCl SDF
Smiles	CCC1C(COC1=O)CC2=CN=CN2C.Cl
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 49 mg/mL ( (200.22 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 49 mg/mL

Ethanol : 49 mg/mL

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In vivo
Batch:

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In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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