Home DNA Damage/DNA Repair ADC Cytotoxin inhibitor - DNA/RNA Synthesis inhibitor Psoralen For research use only.

Psoralen

Synonyms: Psoralene, Ficusin, Furocoumarin

Psoralen (Psoralene, Ficusin, Furocoumarin) is a naturally occurring furocoumarin that intercalates with DNA, inhibiting DNA synthesis and cell division.

Psoralen Chemical Structure

Psoralen Chemical Structure

CAS: 66-97-7

Selleck's Psoralen has been cited by 1 publication

Purity & Quality Control

Batch: Purity: 99.94%
99.94

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Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LoVo Cytotoxicity assay Cytotoxicity against LoVo (human intestinal adenocarcinoma) cell line; Range 0.4-16 uM, IC50=16 μM 15837311
HL-60 Cytotoxicity assay Cytotoxicity against HL-60 (human fibrosarcoma) cell line; Range 0.4-16 uM, IC50=16 μM 15837311
HT-29 cells Cytotoxicity assay 96 h Cytotoxicity against human HT-29 cells after 96 hrs by MTT assay, IC50=41.7 μM 23746477
HCT116 cells Cytotoxicity assay 96 h Cytotoxicity against human HCT116 cells after 96 hrs by MTT assay, IC50=45.6 μM 23746477
Click to View More Cell Line Experimental Data

Biological Activity

Description Psoralen (Psoralene, Ficusin, Furocoumarin) is a naturally occurring furocoumarin that intercalates with DNA, inhibiting DNA synthesis and cell division.
In vitro
In vitro Psoralen inhibits the proliferation of MCF-7/ADR cells as shown by G0/G1 phase arrest rather than encouraging apoptosis. Psoralen reverses MDR(multidrug resistance) through inhibiting ATPase activity rather than reducing P-gp expression. Psoralen inhibits the migration abilities of MCF-7/ADR cells by repressing EMT possibly through inhibiting the activation of NF-κB. Psoralens are photoactive compounds that readily alkylate DNA when activated by longwave ultraviolet light. Proliferation has been significantly promoted in MCF-7/ADR cells treated with low concentration of psoralen (<10.75 µM) and inhibited with high concentration(>21.5 µM). Psoralen can inhibit metastasis of breast cancer. Psoralen mediates a variety of cell processes including cell death, proliferation, inflammation and migration[1].
Cell Research Cell lines Human breast cancer MCF-7/ADR cells, MCF-10A, and MCF-7 cells
Concentrations 0, 21.5, 43.0, 64.5, 86.0, 107.5 µM
Incubation Time 48 h
Method

The effects of psoralen on cell proliferation are measured by MTT assay. MCF-10A and MCF-7/ADR cells are cultured in 96-well plates at a cell density of 2×104 cells per well for 48 h. The medium is then removed and replaced by fresh medium containing different concentrations of psoralen (0, 21.5, 43.0, 64.5, 86.0, 107.5 μM) for 48 h. Cells in the negative control group are incubated with RPMI-1640 culture medium supplemented with 0.1% dimethyl sulfoxide (DMSO). Cells are incubated with 10 µL MTT (5 mg/mL) for 4 h, and then discarded the medium and added 200 µL DMSO. The spectrophotometric absorbance is measured at 490 nm with enzyme-labeling instrument after the crystals were fully dissolved.
In Vivo
In vivo Psoralen has been characterized as a tumor suppressor in various tumors[1]. Psoralen ameliorates sex hormone deficiency-induced osteoporosis in female and male mice. It has antiosteoporosis effect in ovariectomy-induced osteoporotic rats via stimulating osteoblastic differentiation from bone mesenchymal stem cells[2].
Animal Research Animal Models ICR mice
Dosages 10 mg/kg and 20 mg/kg
Administration intragastrically

Chemical lnformation & Solubility

Molecular Weight 186.16 Formula

C11H6O3
CAS No. 66-97-7 SDF Download Psoralen SDF
Smiles C1=CC(=O)OC2=CC3=C(C=CO3)C=C21
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 37 mg/mL ( (198.75 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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