Home Proteases DPP inhibitor - Aminopeptidase inhibitor - Apoptosis related activator Puromycin aminonucleoside For research use only.

Puromycin aminonucleoside

Synonyms: NSC 3056, PAN, Stylomycin aminonucleoside, ARDMA, SAN

Puromycin aminonucleoside (NSC 3056, PAN, Stylomycin aminonucleoside, ARDMA, SAN), the aminonucleoside portion of the antibiotic puromycin, is a reversible inhibitor of dipeptidyl-peptidase II and cytosol alanyl aminopeptidase that induces apoptosis in mesangial cells (MCs) accompanied by declined cell viability and enhanced inflammatory response.

Puromycin aminonucleoside Chemical Structure

Puromycin aminonucleoside Chemical Structure

CAS: 58-60-6

Selleck's Puromycin aminonucleoside has been cited by 3 publications

Purity & Quality Control

Batch: S963101 DMSO] 59 mg/mL] false] Water] 30 mg/mL] false] Ethanol] Insoluble] false Purity: 99.91%
99.91

Other DPP Products

Related compound libraries

Choose Selective DPP Inhibitors

Biological Activity

Description Puromycin aminonucleoside (NSC 3056, PAN, Stylomycin aminonucleoside, ARDMA, SAN), the aminonucleoside portion of the antibiotic puromycin, is a reversible inhibitor of dipeptidyl-peptidase II and cytosol alanyl aminopeptidase that induces apoptosis in mesangial cells (MCs) accompanied by declined cell viability and enhanced inflammatory response.
Targets
DPP-2 [1] cytosol alanyl aminopeptidase [1]
In vitro
In vitro

Puromycin aminonucleoside (PAN) induces MC apoptosis accompanied by the declined cell viability and enhanced inflammatory response. In PAN-treated MCs, ERRα overexpression further aggravates PAN-induced apoptosis.[1]
Cell Research Cell lines mouse mesangial cells
Concentrations 50 μg/ml, 100 μg/ml
Incubation Time 24 h
Method

For apoptosis assay, mesangial cells are treated with PAN for 24 h at the indicated concentration. After treatment, cells are stained with FITC-Annexin V and PI according to the manufacturer’s protocol. Cell apoptosis is determined using a BD FACS Calibur flow cytometer and data is analyzed with Flowjo software.
In Vivo
In vivo

In agreement with the in vitro study, increased ERRα expression is observed in line with enhanced apoptotic response in renal cortex from PAN-treated rats.[1]
Animal Research Animal Models male Sprague-Dawley rats
Dosages 150 mg/kg
Administration IV

Chemical lnformation & Solubility

Molecular Weight 294.31 Formula

C12H18N6O3
CAS No. 58-60-6 SDF --
Smiles CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)N)O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 59 mg/mL ( (200.46 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 30 mg/mL

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Puromycin aminonucleoside | Puromycin aminonucleoside supplier | purchase Puromycin aminonucleoside | Puromycin aminonucleoside cost | Puromycin aminonucleoside manufacturer | order Puromycin aminonucleoside | Puromycin aminonucleoside distributor