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Rhamnose

Name: Rhamnose
Brand: Selleck Chemicals
SKU: E0245

Synonyms: L-Rhamnose

Catalog No.E0245 Purity: 99.36%

Rhamnose(L-Rhamnose) is an important 6-deoxy sugar present in many natural products, glycoproteins, and structural polysaccharides.

Rhamnose Chemical Structure

CAS: 3615-41-6

Purity & Quality Control

Batch: E024501 DMSO] 33 mg/mL] false] ] ] false] ] ] false Purity: 99.36%

99.36

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Biological Activity

Description	Rhamnose(L-Rhamnose) is an important 6-deoxy sugar present in many natural products, glycoproteins, and structural polysaccharides.

In vitro
In vitro	The polymeric copies of a rhamnose motif form glycopolymers (ARGPs), which can bind endogenous antirhamnose antibodies present in human serum, thus attract antibodies from human serum to the target cell surface.^[1]
Cell Research	Cell lines	Jurkat T Cells
	Concentrations	1 mg/mL
	Incubation Time	1 h
	Method	Jurkat T cells are resuspended in the RPMI medium without FBS at a final concentration of 350000 cells/mL and placed on ice. After 30 min of incubation on ice, 1 mL of cold cell suspension is incubated for 1 h on ice in the dark with 40 μL of a 1 mg/mL stock solution of the CholA-p(RhamEAmAIBN) polymer in PBS. As a negative control, 1 mL of cold cell suspension is incubated for 1 h on ice in the dark with 40 μL of a 1 mg/mL stock solution of the CholA-p(GalEAmAIBN) polymer in PBS. After the incubation, cells are washed twice with PBS by centrifugation for 3 min at 500 G. Afterward, cells are resuspended in 500 μL of flow incubation buffer (1% bovine serum albumin, 5 mM Na2EDTA in PBS) containing 10% of human serum and are then incubated for 30 min in the dark on ice. After this incubation period, cells are centrifuged at 500 G for 3 min and washed once with PBS. Cells are spun down again at 500 G for 3 min and incubated during 1 h in the dark at room temperature with 500 μL of goat antihuman IgG secondary antibody bound to Alexa 647 at a final concentration of 4 μL/mL and 1% human albumin serum in PBS. Incubation is followed by two washing steps with PBS by centrifugation at 500 G for 5 min. Cells are imaged using stochastic optical reconstruction microscopy.

In Vivo
In vivo	Rhamnose(Rha) conjugated immunogens induces high titers of anti-Rha antibodies in wildtype mice, which can replace α1,3galactosyltransferase knockout (α1,3GT KO) mice in such antigen/antibody-mediated vaccine design when developing cancer immunotherapies.^[2]
Animal Research	Animal Models	BALB/c mice
	Dosages	30 µg
	Administration	s.c.

References

Chemical lnformation & Solubility

Molecular Weight	164.16	Formula	C₆H₁₂O₅
CAS No.	3615-41-6	SDF	--
Smiles	CC(O)C(O)C(O)C(O)C=O
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 33 mg/mL ( (201.02 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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% DMSO + % + % Tween 80 + % ddH₂O

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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