Home Metabolism Mitochondrial Metabolism inhibitor - Dyes chemical Rhodamine 6G For research use only.

Rhodamine 6G

Synonyms: R6G, Basic Red 1, Rhodamine 590

Rhodamine 6G (R6G, Basic Red 1, Rhodamine 590) is a fluorescence tracer that binds to mitochondria, thus reducing the intact mitochondria number and inhibiting mitochondrial metabolic activity.

Rhodamine 6G Chemical Structure

Rhodamine 6G Chemical Structure

CAS: 989-38-8

Purity & Quality Control

Batch: S359301 DMSO] 96 mg/mL] false] Ethanol] 96 mg/mL] false] Water] Insoluble] false Purity: 98%
98

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Biological Activity

Description Rhodamine 6G (R6G, Basic Red 1, Rhodamine 590) is a fluorescence tracer that binds to mitochondria, thus reducing the intact mitochondria number and inhibiting mitochondrial metabolic activity.
In vitro
In vitro

1.Preparation of Rhodamine 6G working solution
1.1Preparation of the stock solution
Dissolve 1 mg Rhodamine 6G in 525 μL DMSO to obtain 5 mM of stock solution.
1.2Preparation of Rhodamine 6G working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-20 μM of working solution.
Note: Please adjust the concentration of Rhodamine 6G working solution according to the actual situation.
2.Cell staining
2.1 Suspension cells (6-well plate)
a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
b.Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d.Wash twice with PBS, 5 minutes each time.
e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a.Culture adherent cells on sterile coverslips.
b.Remove the coverslip from the medium and aspirate excess medium.
c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 30-60 minutes.
d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.
Note: If detection by flow cytometry, cells need to be resuspended before staining.

Chemical lnformation & Solubility

Molecular Weight 479.01 Formula

C28H31ClN2O3
CAS No. 989-38-8 SDF --
Smiles [Cl-].CCNC1=CC2=C(C=C1C)C(=C3C=C(C)C(C=C3O2)=[NH+]CC)C4=CC=CC=C4C(=O)OCC
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 96 mg/mL ( (200.41 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 96 mg/mL

Water : Insoluble


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In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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