Home MAPK ERK inhibitor - JNK inhibitor - Akt inhibitor - mTOR inhibitor - NF-κB inhibitor Astragaloside IV For research use only.

Astragaloside IV

Synonyms: AST-IV, AS-IV

Astragaloside IV (AST-IV, AS-IV) is a bioactive saponin first isolated from the dried plant roots of the genus Astragalus, which is used in traditional Chinese medicine. It has various effect on the cardiovascular, immune, digestive, and nervous systems. AS-IV suppresses activation of p-Akt, p-mTOR, p-NF-κB and p-Erk1/2.

Astragaloside IV Chemical Structure

Astragaloside IV Chemical Structure

CAS: 84687-43-4

Selleck's Astragaloside IV has been cited by 3 publications

Purity & Quality Control

Batch: Purity: 100.00%
100.00

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Biological Activity

Description Astragaloside IV (AST-IV, AS-IV) is a bioactive saponin first isolated from the dried plant roots of the genus Astragalus, which is used in traditional Chinese medicine. It has various effect on the cardiovascular, immune, digestive, and nervous systems. AS-IV suppresses activation of p-Akt, p-mTOR, p-NF-κB and p-Erk1/2.
Targets
JNK [2] ERK [2] Akt [2] mTOR [2] NF-κB [2]
In vitro
In vitro Astragaloside IV probably alleviates leptin resistance by comprehensive modulation of ObR/STAT3 signaling transduction in neuronal cells[2].
Cell Research Cell lines SH-SY5Y cells
Concentrations 0, 25, 50 and 100 μM
Incubation Time 24 h
Method

Neuronal cell line SH-SY5Y cells are maintained in DMEM/F-12 medium supplemented with 10% fetal bovine serum (FBS) at 37 °C in a humidified incubator supplied with 5% CO2. After seeded in 6-well plate at 5 × 105 cells/ml and cultured without FBS overnight, the cells are treated with ASI (0, 25, 50 and 100 μM) for 24 h. Thereafter, they are harvested with CelLyticTM MT mammalian tissue lysis reagent supplemented with protease and phosphatase inhibitors followed by sonication, and stored at −80℃ for further western blotting analysis.
In Vivo
In vivo Administration of astragaloside IV (AS-IV) reduces albuminuria, ameliorates changes in the glomerular and tubular pathology, and decreases urinary NAG, NGAL, and TGF-β1 in db/db mice. AS-IV also attenuates the diabetes-related activation of Akt/mTOR, NFκB, and Erk1/2 signaling pathways without causing any detectable hepatotoxicity. AS-IV lowers blood glucose levels in streptozotocin (STZ)-induced diabetic models. It prevents body weight gain and reduced accumulation of fat tissues in HFD fed mice. Astragaloside IV actively participates in the modulation of energy homeostasis[1][2].
Animal Research Animal Models Six-weeks old male C57BL/6 mice
Dosages 25 mg/kg/d
Administration i.p.

Chemical lnformation & Solubility

Molecular Weight 784.97 Formula

C41H68O14
CAS No. 84687-43-4 SDF Download Astragaloside IV SDF
Smiles CC1(C(CCC23C1C(CC4C2(C3)CCC5(C4(CC(C5C6(CCC(O6)C(C)(C)O)C)O)C)C)OC7C(C(C(C(O7)CO)O)O)O)OC8C(C(C(CO8)O)O)O)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (127.39 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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