Home Immunology & Inflammation ROS inhibitor Sodium Nitrite For research use only.

Sodium Nitrite

Sodium nitrite is a myeloperoxidase inhibitor with IC50 of 1.3 μM.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.

Sodium Nitrite Chemical Structure

Sodium Nitrite Chemical Structure

CAS: 7632-00-0

Purity & Quality Control

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Biological Activity

Description Sodium nitrite is a myeloperoxidase inhibitor with IC50 of 1.3 μM.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.
Targets
myeloperoxidase [1]
1.3 μM(Kd)
In vitro
In vitro Sodium nitrite is well known for its role in inhibiting the growth of Clostridium botulinum by inhibiting iron-sulfur clusters essential to energy metabolism. Sodium nitrite slows chlorination by univalently reducing myeloperoxidase to an inactive form and as a consequence is oxidized to nitrogen dioxide. Myeloperoxidase oxidizes free tyrosine to tyrosyl radicals that exchange with tyrosyl residues in peptides. These peptide radicals then couple with nitrogen dioxide to form 3-nitrotyrosyl residues. With neutrophils, myeloperoxidase-dependent nitration required a high concentration of nitrite (1 mM), is doubled by tyrosine, and increases 4-fold by superoxide dismutase. Superoxide is likely to inhibit nitration by reacting with nitrogen dioxide and/or tyrosyl radicals. [1] Sodium nitrite results in intoxication of white mice with decrease of red cell superoxide dismutase (SOD) and catalase activity. The total activity of glucose-6-phosphate dehydrogenase and dehydrogenase of 6-phosphogluconate as well as the activity of glutathione reductase are higher in the group of mice that receive sodium nitrite in comparison with the control group. [3]
Kinase Assay H2O2 uptake assay
Reactions are started by adding 10 nM myeloperoxidase to 30 μM H 2 O 2 and varying concentrations of nitrite, in 100 mM phosphate buffer, pH 7.4, containing 20 μM DTPA. Steady state rates of H 2 O 2 loss at 21°C are calculated over the first minute. Data are means and ranges of at least duplicate experiments.
Cell Research Cell lines Jurkat cells
Concentrations ~25 μM
Incubation Time 36 h
Method

cell density
In Vivo
In vivo Peak plasma levels of nitrite are achieved in both sexes approximately 30 min after oral exposure. The model predicts that 10% of the hemoglobin is oxidized to the ferric form after oral doses of 15.9 mg/kg in male rats and 11.0 mg/kg in female rats and after intravenous doses of 8.9 and 7.1 mg/kg in male and female rats, respectively. The t1/2 for recovery from methemoglobinemia is 60 to 120 min depending on dose and route of administration. Replacement of the Vmax of methemoglobin reductase with a value representative of humans predicted a 10% methemoglobinemia following an intravenous dose of 5.8 mg/kg, in close agreement with an observed value of 5.7 mg/kg for humans. [2]
Animal Research Animal Models Twelve-week old Fischer 344 rats
Dosages 20 mg/kg
Administration tail vein injection
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT04354051 Completed Cardiovascular Diseases|Vasodilation|Hypoxia University of East Anglia|Royal Brompton & Harefield NHS Foundation Trust July 1 2018 Phase 1|Phase 2
NCT02987088 Completed Out-Of-Hospital Cardiac Arrest University of Washington December 2016 Phase 1
NCT01715883 Terminated Primary Graft Dysfunction Gladwin Mark MD|University of Pittsburgh October 2011 Phase 2
NCT01316796 Completed Sickle Cell Anemia|Sickle Cell Disease|Chronic Hemolytic Disorders National Heart Lung and Blood Institute (NHLBI)|National Institutes of Health Clinical Center (CC) March 15 2011 Phase 1
NCT00873015 Completed Subarachnoid Hemorrhage Hope Pharmaceuticals|National Institute of Neurological Disorders and Stroke (NINDS) April 2010 Phase 2
NCT01033227 Terminated Sickle Cell Disease Children''s Hospital Los Angeles|Hope Pharmaceuticals December 2009 Phase 1|Phase 2

Chemical lnformation & Solubility

Molecular Weight 69 Formula

HNO2.Na
CAS No. 7632-00-0 SDF Download Sodium Nitrite SDF
Smiles [Na+].[O-]N=O
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In vivo
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