Home Others Dyes inhibitor Thiazolyl Blue For research use only.

Thiazolyl Blue

Synonyms: MTT, Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium

Thiazolyl Blue (MTT, Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium) is a cell-permeable and positively charged colorimetric agent that is widely used to detect reductive metabolism in cells and to measure cell proliferation, cytotoxicity, and apoptosis. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells.

Thiazolyl Blue Chemical Structure

Thiazolyl Blue Chemical Structure

CAS: 298-93-1

Selleck's Thiazolyl Blue has been cited by 4 publications

Purity & Quality Control

Batch: S682101 DMSO] 21 mg/mL] false] Ethanol] 7 mg/mL] false] Water] 2 mg/mL] false Purity: 99.86%
99.86

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Biological Activity

Description Thiazolyl Blue (MTT, Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium) is a cell-permeable and positively charged colorimetric agent that is widely used to detect reductive metabolism in cells and to measure cell proliferation, cytotoxicity, and apoptosis. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells.
In vitro
In vitro

1.Preparation of MTT working solution
Dissolve MTT by PBS to obtain 5 mg/mL of MTT.
2.Cell proliferation detection ( 96-well plates)
2.1Inoculated cells:Prepared single cell suspension to prepared with culture medium containing 10% FBS and inoculated into 96-well plate with 1000-10000 cells per well, volume of 100 uL per well.
2.2Incubated cells: 37℃. 5% CO2, Incubate for 24-72 h.
2.3Add 10 μL MTT in per well and incubate for 4 h, Discard the supernatant, For suspended cells, need centrifuge firstly.
2.4Add 100 μL DMSO, shock 10 min to dissolve the crystal completely
2.5Monitor the absorbance increase with an absorbance plate reader at OD = 562 nm.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06197308 Not yet recruiting Primary Sclerosing Cholangitis University of Minnesota January 1 2024 Early Phase 1
NCT05516472 Not yet recruiting Kidney Stones Joshua Stern|University of Minnesota|Albert Einstein College of Medicine|Litholink Corporation|Intermountain Health Care Inc. June 2023 Early Phase 1
NCT05578872 Recruiting Melanoma (Skin)|Cutaneous Melanoma|Adult Disease|Advanced Solid Tumor|Metastatic Melanoma Anaveon AG December 16 2022 Phase 1|Phase 2
NCT05456477 Completed PreDiabetes Midwest Center for Metabolic and Cardiovascular Research|National Cattlemen''s Beef Association October 7 2022 Not Applicable
NCT04323566 Enrolling by invitation Obsessive-Compulsive Disorder|Obsessive-Compulsive Behavior|Schizophrenia|Delusional Disorder|Brief Psychotic Disorder|Schizo Affective Disorder|Other Psychoses|Unspecified Psychosis Uppsala University Hospital|Uppsala University May 1 2022 Phase 2
NCT05035511 Recruiting Transcranial Direct Current Stimulation|Autistic Disorders Spectrum|Electroencephalography|Machine Learning The Hong Kong Polytechnic University|Chinese University of Hong Kong January 5 2022 Not Applicable

Chemical lnformation & Solubility

Molecular Weight 414.32 Formula

C18H16BrN5S
CAS No. 298-93-1 SDF --
Smiles CC1=C(SC(=N1)[N+]2=NC(=NN2C3=CC=CC=C3)C4=CC=CC=C4)C.[Br-]
Storage (From the date of receipt) 3 years -20°C(in the dark) powder

In vitro
Batch:

DMSO : 21 mg/mL ( (50.68 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 7 mg/mL

Water : 2 mg/mL


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In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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