Home Others Dyes chemical Trypan Blue For research use only.

Trypan Blue

Synonyms: Visionblue, Diphenyl Blue, Direct Blue 14

Trypan Blue (Visionblue, Diphenyl Blue, Direct Blue 14) is a colorimetric dye that stains dead cells with a blue color easily observed using light microscopy at low resolution.

Trypan Blue Chemical Structure

Trypan Blue Chemical Structure

CAS: 72-57-1

Selleck's Trypan Blue has been cited by 1 publication

Purity & Quality Control

Batch: S689001 DMSO] 100 mg/mL] false] Water] 100 mg/mL] false] Ethanol] Insoluble] false Purity: 99%
99

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Biological Activity

Description Trypan Blue (Visionblue, Diphenyl Blue, Direct Blue 14) is a colorimetric dye that stains dead cells with a blue color easily observed using light microscopy at low resolution.
In vitro
In vitro

1.1 Preparation of the stock solution
Dissolve 10 mg of Trypan Blue in 100 mL 0.85% Nacl to obtain 0.4% Trypan Blue.
Note: It is recommended to store the stock solution at -20℃ -80℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Trypan Blue working solution
Dilute the stock solution in 0.85% Nacl to obtain 0.04% of Trypan Blue working solution.
Note: Please adjust the concentration of Trypan Blue working solution according to the actual situation.
Cell staining
2.1 For suspension cells, centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells, discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of rypan Blue working solution, and then incubate at room temperature for 5 minutes.
2.3 Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05847387 Not yet recruiting Corneal Transplant Failure|Corneal Endothelial Cell Loss Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia November 1 2023 --
NCT06167928 Recruiting Osteoarthritis Keele University|Alder Hey Children''s NHS Foundation Trust|Birmingham Women''s and Children''s NHS Foundation Trust|Norfolk and Norwich University Hospitals NHS Foundation Trust|Royal National Orthopaedic Hospital NHS Trust|Scottish National Blood Transfusion Service|NHS Blood and Transplant|University of Birmingham|Robert Jones and Agnes Hunt Orthopaedic and District NHS Trust|The Royal Orthopaedic Hospital NHS Trust October 8 2018 --
NCT01385878 Unknown status Cataract Iladevi Cataract and IOL Research Center January 2011 Not Applicable

Chemical lnformation & Solubility

Molecular Weight 960.81 Formula

C34H24N6Na4O14S4
CAS No. 72-57-1 SDF --
Smiles [Na+].[Na+].[Na+].[Na+].CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(O)C4=C(N)C=C(C=C4C=C3[S]([O-])(=O)=O)[S]([O-])(=O)=O)C)N=NC5=C(O)C6=C(N)C=C(C=C6C=C5[S]([O-])(=O)=O)[S]([O-])(=O)=O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (104.07 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 100 mg/mL

Ethanol : Insoluble


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In vivo
Batch:

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Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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