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Tween 80

Name: Tween 80
Brand: Selleck Chemicals
SKU: S6702
Rating: 5 (28 reviews)

Synonyms: Polysorbate 80

Catalog No.S6702 Purity: 99%

Tween 80 is a polysorbate surfactant used for selective protein extraction and the isolation of nuclei from mammalian cell lines. Tween 80 has been used in drug vehicle solutions for specific injections in mice and rats. It has been used for the determination of esterase activity in bacterial cells.

Tween 80 Chemical Structure

CAS: 9005-65-6

Selleck's Tween 80 has been cited by 28 publications

Purity & Quality Control

Batch: Purity: 99%

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Biological Activity

Description	Tween 80 is a polysorbate surfactant used for selective protein extraction and the isolation of nuclei from mammalian cell lines. Tween 80 has been used in drug vehicle solutions for specific injections in mice and rats. It has been used for the determination of esterase activity in bacterial cells.

References

Chemical lnformation & Solubility

Molecular Weight		Formula
CAS No.	9005-65-6	SDF	--
Density	1.08 g/mL at 20 °C
Smiles	CCCCCCCCC=CCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
Storage (From the date of receipt)	2 years room temperature liquid	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	2 years room temperature liquid		1 month-20°Cin solvent

In vitro
Batch:

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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