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Vitamin C

Name: Vitamin C
Brand: Selleck Chemicals
SKU: S3114
Rating: 5 (12 reviews)

Synonyms: L-Ascorbic acid

Catalog No.S3114 Purity: 99.97%

Vitamin C (L-Ascorbic acid) is a water-soluble vitamin indicated for the prevention and treatment of scurvy.

Vitamin C Chemical Structure

CAS: 50-81-7

Selleck's Vitamin C has been cited by 12 publications

Purity & Quality Control

Batch: Purity: 99.97%

99.97

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Biological Activity

Description	Vitamin C (L-Ascorbic acid) is a water-soluble vitamin indicated for the prevention and treatment of scurvy.

In vitro
In vitro	Ascorbate when combined with copper rapidly reduces the viscosity of DNA solutions and has exhibited some carcinostatic effects on transplanted sarcoma 180 tumours in mice. ^[1] Exposure of HL-60 cells to increasing concentrations of H2O2 results in dose-dependent intracellular accumulation of peroxides, which is accompanied by a decrease in intracellular ascorbic acid and an increase in apoptosis. Ascorbic acid loading also provides strong protection from apoptosis associated with serum- and glucose-free culture of HL-60 cells. ^[2] Vitamin C is found to protect GSH-depleted HL-60 cells in a dose-dependent manner. Vitamin C inhibits the formation of ROS in both normal and GSH-depleted cells. ^[3] Ascorbate (1 mM) generates 161 mM H2O2 in Dulbecco's modified Eagle's medium and induces apoptosis in 50% of HL60 cells, whereas in RPMI 1640 only 83 mM H2O2 is produced and no apoptosis is detected. ^[4]
Cell Research	Cell lines	Fibroblasts
	Concentrations	100 μM
	Incubation Time	2 h
	Method	Cells were treated with indicated concentration of drug for 2 h.

In Vivo
In vivo	Treatment of rats with Vitamin C 10 min prior to the cisplatin inhibits cisplatin-mediated damage. The enhanced serum creatinine levels which are indicative of renal injury shows a significant reduction with the three doses of Vitamin C tested. ^[5]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT06032234	Recruiting	Healthy	Isabelle Herter-Aeberli\|Berner Fachhochschule\|Swiss Federal Institute of Technology	September 12 2023	--
NCT05810233	Recruiting	Rhinitis Allergic\|Allergy	National University of Malaysia	July 15 2023	Not Applicable
NCT05975125	Recruiting	Vitamin C Anemia\|Pregnancy Anemia	Rajavithi Hospital	June 28 2023	Not Applicable
NCT05501548	Recruiting	Prostate Cancer\|Castration-resistant Prostate Cancer	Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins\|AstraZeneca\|McGuff Pharmaceuticals Inc.\|The Marcus Foundation	June 30 2023	Phase 2
NCT05788952	Not yet recruiting	Endometriosis	Universidade Nova de Lisboa\|Academia CUF	June 1 2023	--
NCT04505878	Withdrawn	Primary Graft Dysfunction\|Lung Transplant; Complications	University of Wisconsin Madison	April 2023	Phase 2

References

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Chemical lnformation & Solubility

Molecular Weight	176.12	Formula	C₆H₈O₆
CAS No.	50-81-7	SDF	Download Vitamin C SDF
Smiles	C(C(C1C(=C(C(=O)O1)O)O)O)O
Storage (From the date of receipt)	3 years -20°C powder (seal)	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder (seal)		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 35 mg/mL ( (198.72 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 35 mg/mL

Ethanol : Insoluble

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In vivo
Batch:

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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% DMSO + % + % Tween 80 + % ddH₂O

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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