Home NF-κB NF-κB inhibitor - Autophagy activator - Apoptosis related activator DHA (Dihydroartemisinin) For research use only.

DHA (Dihydroartemisinin)

Synonyms: Dihydroqinghaosu, β-Dihydroartemisinin, Artenimol

DHA (Dihydroartemisinin) is a semi-synthetic derivative of artemisinin and isolated from the traditional Chinese herb Artemisia annua. Dihydroartemisinin induces autophagy and apoptosis by suppressing NF-κB activation.

DHA (Dihydroartemisinin) Chemical Structure

DHA (Dihydroartemisinin) Chemical Structure

CAS: 71939-50-9

Selleck's DHA (Dihydroartemisinin) has been cited by 28 Publications

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Purity & Quality Control

Batch: Purity: 99%
99

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Biological Activity

Description DHA (Dihydroartemisinin) is a semi-synthetic derivative of artemisinin and isolated from the traditional Chinese herb Artemisia annua. Dihydroartemisinin induces autophagy and apoptosis by suppressing NF-κB activation.
Targets
NF-κB [4]
In vitro
In vitro

Dihydroartemisinin (DHA) inhibits the growth of certain cancer cell lines and xenograft tumors such as leukemia, glioma, fibrosarcoma, and breast, cervical, ovarian, lung, oral and pancreatic cancer. DHA inhibits cell and tumor growth by modulating various tumor-suppressive pathways, such as inhibiting cell proliferation and inducing apoptosis through regulation of proliferation- and apoptosis-related proteins.DHA inhibits the proliferation and viability of cells in a dose-dependent manner and induces apoptosis.DHA-mediated cytotoxicity is tumor selective. The endoperoxide bridge of DHA is reportedly essential for its cytotoxicity because it reacts with intracellular ferrous iron to generate reactive oxygen species or carbon-centered radicals, leading to cytotoxicity[1].
Cell Research Cell lines pancreatic cancer cell line BxPc3-RFP
Concentrations 2.5, 10, 40, or 80 μM
Incubation Time 24, 48, and 72 h
Method

BxPc3-RFP cells (3.5×104cells/well) were seeded in poly D-lysine-coated black, μClear 96-well plates with 0.2 ml medium. After 24 h, the cells were treated with dimethyl sulfoxide (DMSO) (control) or different concentrations (2.5, 10, 40, or 80 μM) of DHA dissolved in DMSO for 24, 48, and 72 h. At each time point, the fluorescence intensity emitted from cells was measured.
Experimental Result Images Methods Biomarkers Images PMID
Western blot p-STAT3 / p-JAK2 MMP-2 / MMP-9 E-cadherin / N-cadherin / Vimentin / Twist / Slug / Snail 29434895
Immunofluorescence PDGFRα 29387451
Growth inhibition assay Cell viability 29114376
In Vivo
In vivo

DHA significantly inhibited HCC cell growth in vitro and in vivo via inducing G2/M cell cycle arrest and apoptosis[2].

DHA has been shown in the rat whole embryo culture (WEC) to primarily affect primitive red blood cells (RBCs) causing subsequent tissue damage and dysmorphogenesis[3].
Animal Research Animal Models females, BALB/cA Jcl-nu/nu mice
Dosages 25 mg/kg 
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03402789 Not yet recruiting Amblyopia Johns Hopkins University January 1 2024 Phase 1|Phase 2
NCT05750459 Recruiting Plasmodium Falciparum Infection National Institute of Allergy and Infectious Diseases (NIAID) November 29 2023 Phase 4
NCT04575753 Not yet recruiting Delirium Université Libre de Bruxelles|CHU de Charleroi October 7 2023 --

Chemical lnformation & Solubility

Molecular Weight 284.35 Formula

C15H24O5
CAS No. 71939-50-9 SDF Download DHA (Dihydroartemisinin) SDF
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 14 mg/mL ( (49.23 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 7 mg/mL

Water : Insoluble


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In vivo
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