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Sulfanilamide

Sulfanilamide (Sulphanilamide) is a competitive inhibitor for bacterial enzyme dihydropteroate synthetase with IC50 of 320 μM.

Sulfanilamide Chemical Structure

Sulfanilamide Chemical Structure

CAS: 63-74-1

Purity & Quality Control

Batch: S168501 DMSO] 34 mg/mL] false] Ethanol] 17 mg/mL] false] Water] Insoluble] false Purity: 99.95%
99.95

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Biological Activity

Description Sulfanilamide (Sulphanilamide) is a competitive inhibitor for bacterial enzyme dihydropteroate synthetase with IC50 of 320 μM.
Targets
dihydropteroate synthetase [1]
320 μM
In vitro
In vitro Sulfanilamide containing the sulfonamide functional group displays inhibitory activity for dihydropteroate synthetase partially purified from Escherichia coli which normally uses para-aminobenzoic acid (PABA) for synthesizing the necessary folic acid acting as a coenzyme in the synthesis of purine, pyrimidine and other amino acids, exhibiting an IC 50 of 320 μM for dihydropteroate synthetasea and Km of 2.5 uM for PABA. [1] Sulfanilamide shows IC50 of 286.8 μg/mL for recombinant S. cerevisiae strains with wild-type FOL1 genes, but the single mutation 55Trp to 55Ala or 57Pro to 57Ser within the putative active site of the fungal DHPS confers resistance to Sulfanilamide with IC50 of >800 μg/mL. [2] Sulfanilamide moderately inhibits the growth of bacterial cells harboring plasmodium falciparum pKOS-pfPPPK-DHPS (His) with IC50 of 380 uM. [3]
In Vivo
In vivo Administration of Sulfanilamide with the dosage of 100 mg/kg/day is effective in the prevention of P. carinii infection in the immunosuppressed rat model. When the dosage of sulfaguanidine and Sulfanilamide reduced to 10 mg/kg/day, breakthrough P. carinii infection occurs in the rats. [4]
Animal Research Animal Models Male Sprague-Dawley rats
Dosages 100 mg/kg
Administration Orally taken every day

Chemical lnformation & Solubility

Molecular Weight 172.2 Formula

C6H8N2O2S
CAS No. 63-74-1 SDF Download Sulfanilamide SDF
Smiles C1=CC(=CC=C1N)S(=O)(=O)N
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 34 mg/mL ( (197.44 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 17 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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