Home DNA Damage/DNA Repair Topoisomerase inhibitor - Apoptosis related activator - Autophagy activator Topotecan HCl For research use only.

Topotecan HCl

Synonyms: Nogitecan HCl, SKFS 104864A, NSC 609699

Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively. Topotecan HCl induces autophagy and apoptosis.

Topotecan HCl Chemical Structure

Topotecan HCl Chemical Structure

CAS: 119413-54-6

Selleck's Topotecan HCl has been cited by 49 publications

Purity & Quality Control

Batch: Purity: 99.98%
99.98

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Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MDA-MB-435 cells Cytotoxic assay 72 h Cytotoxicity against human MDA-MB-435 cells after 72 hrs by MTT assay, ic50=1 nM 20371183
human Bel7402 cells Cytotoxic assay 96 h Cytotoxicity against human Bel7402 cells after 96 hrs by MTT assay, IC50=1.2 nM 15165144
human HCT8 cells Cytotoxic assay 96 h Cytotoxicity against human HCT8 cells after 96 hrs by MTT assay, IC50=1.5 nM 15165144
human MCF7 cells Cytotoxic assay 96 h Cytotoxicity against human MCF7 cells after 96 hrs by MTT assay, IC50=1.8 nM 15165144
human A549 cells Cytotoxic assay 96 h Cytotoxicity against human A549 cells after 96 hrs by MTT assay, IC50=3.2 nM 15165144
human colon carcinoma SW620 cell Cytotoxic assay Cytotoxic potentiation of Topotecan (TP) by the compound in human colon carcinoma SW620 cell line, IC50=3.2 nM 12408707
human MESSA cells Cytotoxic assay 72 h Cytotoxicity against human MESSA cells after 72 hrs by alamar blue assay, IC50=4 nM 21341674
human BGC823 cells Cytotoxic assay 96 h Cytotoxicity against human BGC823 cells after 96 hrs by MTT assay, IC50=4.3 nM 15165144
human Ketr3 cells Cytotoxic assay 96 h Cytotoxicity against human Ketr3 cells after 96 hrs by MTT assay, IC50=4.9 nM 15165144
LOX cells Cytotoxic assay Cytotoxicity was determined in vitro in LOX cells (melanoma) of human tumor cell lines by using MTT assay, IC50=5 nM 7853331
human A549 cells Function assay 4 h Antitumor activity against human A549 cells after 4 hrs by MTT assay, IC50=5 nM 18207748
human H69 cells Cytotoxic assay 72 h Cytotoxicity against human H69 cells after 72 hrs by alamar blue assay, IC50=6 nM 21341674
human A2780 cells Cytotoxic assay 72 h Cytotoxicity against human A2780 cells overexpressing alpha5beta3 integrin assessed as cell survival after 72 hrs by SRB assay, IC50=6 nM 22959246
human LNCAP cells Cytotoxic assay 72 h Cytotoxicity against human LNCAP cells after 72 hrs by alamar blue assay, IC50=9 nM 21341674
human HOP62 cells Growth inhibition assay Growth inhibition of human HOP62 cells by five-dose growth inhibition assay, IC50=10 nM 25909279
human MCF7 cells Growth inhibition assay Growth inhibition of human MCF7 cells by five-dose growth inhibition assay, IC50=10 nM 25909279
human UACC62 cells Growth inhibition assay Growth inhibition of human UACC62 cells by five-dose growth inhibition assay, IC50=10 nM 25909279
human DU145 cells Growth inhibition assay Growth inhibition of human DU145 cells by five-dose growth inhibition assay, IC50=10 nM 25909279
LoVo cell line Cytotoxic assay Cytotoxic potentiation of Topotecan (TP) by the compound in (human colorectal cancer LoVo cell line, IC50=11.6 nM 12408707
human lymphoblast tumor cell line RPM18402 Cytotoxic assay Cytotoxicity against human lymphoblast tumor cell line RPM18402, IC50=12 nM 12392745
human HL60 cells Function assay Antitumor activity against human HL60 cells by SRB method, IC50=12 nM 19541483
human A375 cells Cytotoxic assay 72 h Cytotoxicity against human A375 cells after 72 hrs by alamar blue assay, IC50=13 nM 21341674
COR-L23/P cell Cytotoxic assay Cytotoxicity measured using the COR-L23 parental (COR-L23/P) human non small cell lung carcinoma cell line, IC50=13.2 nM 11806724
human NCI-H460 cells Proliferation assay 72 h Antiproliferative activity against human NCI-H460 cells after 72 hrs by coulter counter analysis, IC50=16 nM 21783369
human MOLT4 cells Cytotoxic assay 72 h Cytotoxicity against human MOLT4 cells after 72 hrs by MTT assay, IC50=19 nM 24900725
human LoVo cells Cytotoxic assay 72 h Cytotoxicity against human LoVo cells after 72 hrs by MTT assay, IC50=20 nM 20371183
RPM18402 tumor cell line Proliferation assay Concentration required to inhibit cell proliferation in RPM18402 tumor cell line, IC50=20 nM 15482929
human SN12C cells Growth inhibition assay Growth inhibition of human SN12C cells by five-dose growth inhibition assay, GI50=20 nM 25909279
human RPMI8226 cells Cytotoxic assay Cytotoxicity against human RPMI8226 cells by MTT method, IC50=21 nM 19012996
HT-29 cells Cytotoxic assay Cytotoxicity was determined in vitro in HT-29 cells(colon) of human tumor cell lines by using MTT assay, IC50=25 nM 7853331
SK-BR-3 cells Cytotoxic assay In Vitro cytotoxicity against human breast cancer cell line (SK-BR-3), IC50=26 nM 11334569
HeLa cells Growth inhibition assay Growth inhibition of HeLa cells after 4 days, GI50=30 nM 17418582
UACC 62 cells Cytotoxic assay Tested in vitro for cytotoxicity against human tumor cell line UACC 62 (melanoma), GI50=30 nM 10714502
human HCT116 cells Growth inhibition assay Growth inhibition of human HCT116 cells by five-dose growth inhibition assay, GI50=30 nM 25909279
human lung cancer cell line (H128) Cytotoxic assay In Vitro cytotoxicity against human lung cancer cell line (H128), IC50=31 nM 11334569
human MES-SA/Dx5 cells Cytotoxic assay Cytotoxicity against human MES-SA/Dx5 cells overexpressing MDR1 after 72 hrs by alamar blue assay, IC50=33 nM 21341674
human A2780 cells Cytotoxic assay 72 h Cytotoxicity against human A2780 cells after 72 hrs by MTT assay, IC50=33 nM 24900725
human MDA-MB-435 cells Cytotoxic assay 72 h Cytotoxicity against human MDA-MB-435 cells incubated for 72 hrs by MTT assay, GI50=34 nM 22867019
human stomach cancer cell line (MKN45) Cytotoxic assay In Vitro cytotoxicity against human stomach cancer cell line (MKN45), IC50=38 nM 11334569
human KB3-1 cells Cytotoxic assay Cytotoxicity against human KB3-1 cells after MTT assay, IC50=40 nM 18829334
mouse P388 cells Cytotoxic assay Cytotoxicity against mouse P388 cells after MTT assay, IC50=45 nM 18829334
A427 human lung carcinoma Proliferation assay Antiproliferative activity measured against A427 human lung carcinoma, IC50=49 nM 9876111
human U251 cells Function assay 6-24 h Inhibition of topoisomerase-1 in human U251 cells assessed as inhibition of hypoxia-induced HIF-1alpha accumulation in nuclear extract after 6 to 24 hrs by immunoblot analysis, EC50=54 nM 22305612
human colon cancer cell line (WiDr) Cytotoxic assay In Vitro cytotoxicity against human colon cancer cell line (WiDr), IC50=56 nM 11334569
human HepG2 cells Cytotoxic assay 4 days Cytotoxicity against human HepG2 cells after 4 days by MTT assay, IC50=60 nM 18554906
human KB cells Cytotoxic assay 72 h Cytotoxicity against human KB cells after 72 hrs sulforhodamine B colorimetric assay, IC50=62.5 nM 25008456
human HCT8 cells Cytotoxic assay 3 days Cytotoxicity against human HCT8 cells after 3 days by MTT assay, IC50=70 nM 20392545
human GBM2 cells Proliferation assay 72 h Antiproliferative activity against human GBM2 cells assessed as reduction in cell viability incubated for 72 hrs by WST-1 method, IC50=0.13 μM 26355532
SK-MEL-2 cells Cytotoxic assay 72 h Cytotoxicity against human SK-MEL-2 cells after 72 hrs by alamar blue assay, IC50=0.14 μM 21341674
human IGROV1 cells Cytotoxic assay 72 h Cytotoxicity against human IGROV1 cells after 72 hrs by alamar blue assay, IC50=0.16 μM 21341674
human U87 cells Proliferation assay 48 h Antiproliferative activity against human U87 cells assessed as growth inhibition after 48 hrs by MTT assay, IC50=0.16 μM 24826818
human HT1080 cells Proliferation assay 48 h Antiproliferative activity against human HT1080 cells assessed as growth inhibition incubated for 48 hrs by sulforhodamine B assay, IC50=0.18 μM 26408815
human Hep3B cells Proliferation assay 72 h Antiproliferative activity against human Hep3B cells after 72 hrs by XTT assay, GI50=0.22 μM 19796956
human GBM3 cells Proliferation assay 72 h Antiproliferative activity against human GBM3 cells assessed as reduction in cell viability incubated for 72 hrs by WST-1 method, IC50=0.38 μM 26355532
human KBVIN cells Cytotoxic assay 72 h Cytotoxicity against human KBVIN cells after 72 hrs by SRB assay, IC50=0.4 μM 25438769
human GBM1 cells Proliferation assay 72 h Antiproliferative activity against human GBM1 cells assessed as reduction in cell viability incubated for 72 hrs by WST-1 method, IC50=0.46 μM 26355532
human U937 cells Cytotoxic assay 48 h Cytotoxicity against human U937 cells expressing p53 mutant after 48 hrs by SRB assay ， IC50=1.1 μM 19715319
Caco2 Cytotoxicity assay 24 hrs Cytotoxicity against human Caco2 cells after 24 hrs by MTT assay, IC50=0.119μM. 24980118
Jurkat Cytotoxicity assay 24 hrs Cytotoxicity against human Jurkat cells after 24 hrs by MTT assay, IC50=0.127μM. 24980118
A375 Cytotoxicity assay 24 hrs Cytotoxicity against human A375 cells after 24 hrs by MTT assay, IC50=0.162μM. 24980118
HeLa Cytotoxicity assay 24 hrs Cytotoxicity against human HeLa cells after 24 hrs by MTT assay, IC50=0.38μM. 24980118
MDA-MB-231 Cytotoxicity assay 24 hrs Cytotoxicity against human MDA-MB-231 cells after 24 hrs by MTT assay, IC50=0.473μM. 24980118
NCI-H460 Antiproliferative assay 1 hr Antiproliferative activity against human NCI-H460 cells after short term exposure for 1 hr measured after 72 hrs in drug-free medium, IC50=0.61μM. 19530720
HCT8 Antiproliferative assay 48 hrs Antiproliferative activity against human HCT8 cells after 48 hrs by CCK8 assay, IC50=1.875μM. 25481395
KB Antiproliferative assay 48 hrs Antiproliferative activity against human KB cells after 48 hrs by CCK8 assay, IC50=2.282μM. 25481395
SGC7901 Antiproliferative assay 48 hrs Antiproliferative activity against human SGC7901 cells after 48 hrs by CCK8 assay, IC50=2.292μM. 25481395
HepG2 Antiproliferative assay 48 hrs Antiproliferative activity against human HepG2 cells after 48 hrs by CCK8 assay, IC50=4.208μM. 25481395
MDA-MB-231 Apoptosis assay Induction of apoptosis in human MDA-MB-231 cells assessed as p53 phosphorylation at Ser15 by Western blotting analysis 24980118
HeLa Apoptosis assay 0.8 to 1.6 uM 24 hrs Induction of apoptosis in human HeLa cells assessed as inhibition of DNA synthesis at 0.8 to 1.6 uM after 24 hrs by BrdU staining-based ELISA 24980118
MDA-MB-231 Apoptosis assay 0.8 to 1.6 uM 24 hrs Induction of apoptosis in human MDA-MB-231 cells assessed as inhibition of DNA synthesis at 0.8 to 1.6 uM after 24 hrs by BrdU staining-based ELISA 24980118
A375 Apoptosis assay 0.8 to 1.6 uM 24 hrs Induction of apoptosis in human A375 cells assessed as inhibition of DNA synthesis at 0.8 to 1.6 uM after 24 hrs by BrdU staining-based ELISA 24980118
Caco2 Apoptosis assay 0.8 to 1.6 uM 24 hrs Induction of apoptosis in human Caco2 cells assessed as inhibition of DNA synthesis at 0.8 to 1.6 uM after 24 hrs by BrdU staining-based ELISA 24980118
A375 Apoptosis assay Induction of apoptosis in human A375 cells assessed as p53 phosphorylation at Ser15 by Western blotting analysis 24980118
TC32 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for TC32 cells 29435139
DAOY qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for DAOY cells 29435139
SJ-GBM2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells 29435139
A673 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells 29435139
SK-N-MC qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells 29435139
BT-37 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells 29435139
NB-EBc1 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells 29435139
Saos-2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Saos-2 cells 29435139
SK-N-SH qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-SH cells 29435139
NB1643 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB1643 cells 29435139
LAN-5 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for LAN-5 cells 29435139
BT-12 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-12 cells 29435139
Rh18 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh18 cells 29435139
OHS-50 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells 29435139
RD qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for RD cells 29435139
MG 63 (6-TG R) qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for MG 63 (6-TG R) cells 29435139
Rh41 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh41 cells 29435139
NB1643 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for NB1643 cells 29435139
A673 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for A673 cells) 29435139
SK-N-MC qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for SK-N-MC cells 29435139
BT-12 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for BT-12 cells 29435139
LAN-5 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for LAN-5 cells 29435139
DAOY qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for DAOY cells 29435139
NB-EBc1 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for NB-EBc1 cells 29435139
SJ-GBM2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for SJ-GBM2 cells 29435139
BT-37 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for BT-37 cells 29435139
TC32 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for TC32 cells 29435139
MG 63 (6-TG R) qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for MG 63 (6-TG R) cells 29435139
U-2 OS qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for U-2 OS cells 29435139
Rh41 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for Rh41 cells 29435139
RD qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for RD cells 29435139
Rh18 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for Rh18 cells 29435139
Rh30 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for Rh30 cells 29435139
Saos-2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for Saos-2 cells 29435139
OHS-50 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for OHS-50 cells 29435139
SK-N-SH qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for SK-N-SH cells 29435139
SK-N-SH qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Orthogonal 3D viability screen for SK-N-SH cells 29435139
SJ-GBM2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Orthogonal 3D viability screen for SJ-GBM2 cells 29435139
Daoy qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Orthogonal 3D viability screen for Daoy cells 29435139
TC32 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Orthogonal 3D caspase screen for TC32 cells 29435139
TC32 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Orthogonal 3D viability screen for TC32 cells 29435139
MG 63 (6-TG R) qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Orthogonal 3D viability screen for MG 63 (6-TG R) cells 29435139
RD qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Orthogonal 3D viability screen for RD cells 29435139
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Biological Activity

Description Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively. Topotecan HCl induces autophagy and apoptosis.
Features Topotecan is a water-soluble derivative of camptothecin.
Targets
Topo I (DU-145 Luc cells) [1]
(Cell-free assay)		 Topo I (MCF-7 Luc cells) [1]
(Cell-free assay)
2 nM 13 nM
In vitro
In vitro

Stronger drug activity of Topotecan is observed for DU-145 Luc and MCF-7 Luc cells. [1] Topotecan causes cytotoxicity during the course of DNA replication by stabilizing the covalent complex between topoisomerase I and DNA and preventing the religation of enzyme-linked single-strand DNA break. Topotecan stabilizes topoisomerase I/DNA cleavable complexes in radiation-resistant human B-lineage acute lymphoblastic leukemia (ALL) cells, causes rapid apoptotic cell death despite high-level expression of bcl-2 protein, and inhibits ALL cell clonogenic growth in a dose-dependent fashion. [2]
Cell Research Cell lines MCF-7 Luc and DU-145 Luc cells
Concentrations 0 μg/mL - 0.692 μg/mL
Incubation Time 96 hours
Method

Topotecan is dissolved in sterile water to a stock concentration of 1 mg/mL, diluted to 6 μg/mL in cultured medium and then serially diluted 1:4 in opaque, white tissue culture-treated microplates to a final volume of 0.1 mL/well. MCF-7 Luc and DU-145 Luc cells are resuspended in 3×104 cells/mL in DMEM with high glucose containing 10% FBS and 0.5 mg/mL Geneticin; 100 μL of cells are added in each well. Plates are incubated for 4 days at 37 °C in 95% humidity/5% CO2. After incubation, 0.05 mL of 0.1 M HEPES buffer (pH 7.9) containing 50 μg/mL D-luciferin is added to each well. After incubation at room temperature for 10 minutes, the culture microplate is measured in a microplate luminometer and a molecular light imager. Results obtained with the microplate luminometer are calculated using no inhibition control wells without exogenous drug and maximum inhibition control wells containing ATP inhibitor. Results for the molecular light imager are similarly calculated using values obtained with a 5 minutes luminescent imager.
Experimental Result Images Methods Biomarkers Images PMID
Western blot p53 / sesn2 / p-AMPK LC3 / p62 23024792
Growth inhibition assay Cell viability 28544814
In Vivo
In vivo

Animals inoculate s.c. with DU-145 Luc cells and then treated with Topotecan demonstrates significant tumor growth and regression as measured with calipers and luminescent imaging. The correlation coefficient is 0.75 for the control untreated group and 0.93 for the Topotecan-treated group. Similarly, tumor progression and regression are measurable using luminescent imaging for untreated and Topotecan-treated mice inoculated i.p. with MCF-7 Luc cells. [1] Topotecan elicited potent antileukemic activity in severe combined immune-deficiency (SCID) mouse models of human poor prognosis ALL. Topotecan markedly improved event-free survival of SCID mice challenged with otherwise fatal doses of humaln leukemia cells at systemic drug exposure levels. [2] Gliomas preferentially express TRAIL R2 and that treatment with Topotecan significantly up-regulates its expression. [3]
Animal Research Animal Models Mice with MCF-7 Luc or DU-145 Luc cells
Dosages 0.25 mg/mL
Administration Administered via i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT04455139 Terminated Eye Cancer Retinoblastoma Prof. Beck Popovic Maja|University of Lausanne Hospitals November 15 2021 Phase 2
NCT05083000 Unknown status COVID-19 Respiratory Infection National University Hospital Singapore|Christian Medical College Vellore India August 16 2021 Phase 1
NCT04156347 Active not recruiting Retinoblastoma Targeted Therapy Technologies LLC June 16 2021 Phase 1
NCT04428879 Recruiting Retinoblastoma The Hospital for Sick Children June 16 2020 Phase 1

Chemical lnformation & Solubility

Molecular Weight 457.91 Formula

C23H23N3O5.HCl
CAS No. 119413-54-6 SDF Download Topotecan HCl SDF
Smiles CCC1(C2=C(COC1=O)C(=O)N3CC4=CC5=C(C=CC(=C5CN(C)C)O)N=C4C3=C2)O.Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 91 mg/mL ( (198.72 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 91 mg/mL

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
I would like get the compound for mice intraperitoneally injection, What would you recommend to improve solubility? What could I use as solvent (%v?) that is nontoxic (unlike Methanol) and could be injected into mice intraperitoneally?

Answer:
Topotecan HCl is generally prepared by Saline for I.P. administration.

Topoisomerase Signaling Pathway Map

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