Home DNA Damage/DNA Repair Selection Antibiotics for Transfected Cell inhibitor - DNA/RNA Synthesis inhibitor - Antibiotics inhibitor Blasticidin S HCl For research use only.

Blasticidin S HCl

Blasticidin S HCl is a nucleoside antibiotic isolated from Stretomyces girseochromogenes. It acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used to select transfected cells carrying bsr or BSD resistance genes.

Blasticidin S HCl Chemical Structure

Blasticidin S HCl Chemical Structure

CAS: 3513-03-9

Selleck's Blasticidin S HCl has been cited by 22 publications

Purity & Quality Control

Batch: Purity: 99.9%
99.9

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Biological Activity

Description Blasticidin S HCl is a nucleoside antibiotic isolated from Stretomyces girseochromogenes. It acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used to select transfected cells carrying bsr or BSD resistance genes.
Targets
protein synthesis [1] DNA synthesis [1]
In vitro
In vitro

Blasticidin S inhibits protein synthesis by acting on the step of peptide transfer from peptidyl-sRNA to incoming aminoacyl-sRNA. [1]

Blasticidin S also significantly inhibits DNA synthesis independent of their effect on protein synthesis. [2]

Blasticidin S kills the cells more rapidly than G418, which makes blasticidin S-resistance gene (bsr) a selectable marker for mammalian cells. [3]

Besides, Blasticidin S also efficiently binds Cu(II) ions and prevents DNA from metal-induced damage. [4]
Cell Research Cell lines HEK293T cells
Concentrations 5 µg/ml
Incubation Time 7 days
Method

Lentivirus infected cells were maintained in 5 μg/ml blasticidin containing culture medium for 7d, with fresh medium exchange every day.
Experimental Result Images Methods Biomarkers Images PMID
Western blot p-IκBα / T-IκBα / β-actin 16184196
Growth inhibition assay Cell viability 24782309
mRNA blot MGG_00447 / MGG_03736 / MGG_04855 / MGG_09931 / Beta tubulin MGG_00447 / MGG_03736 / MGG_04855 / MGG_09931 / 18s rRNA 26889110
In Vivo
In vivo

Blasticidin S HCl is an antibiotic isolated from Stretomyces girseochromogenes that acts as a protein synthesis inhibitor. It is used to select transfected cells carrying bsr or BSD resistance genes.

Chemical lnformation & Solubility

Molecular Weight 458.9 Formula

C17H27ClN8O5
CAS No. 3513-03-9 SDF Download Blasticidin S HCl SDF
Smiles CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl
Storage (From the date of receipt)

In vitro
Batch:

Water : 92 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble


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In vivo
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