Home Microbiology Antibiotics chemical Apramycin Sulfate For research use only.

Apramycin Sulfate

Synonyms: Ai3-29795

Apramycin Sulfate (Ai3-29795) is an aminoglycoside antibiotic, which binds to the deep groove of the RNA.

Apramycin Sulfate Chemical Structure

Apramycin Sulfate Chemical Structure

CAS: 65710-07-8

Purity & Quality Control

Batch: S425401 Water] 100 mg/mL] false] DMSO] Insoluble] false] Ethanol] Insoluble] false Purity: >97%
97

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Biological Activity

Description Apramycin Sulfate (Ai3-29795) is an aminoglycoside antibiotic, which binds to the deep groove of the RNA.
In vitro
In vitro Apramycin consumption at farm level is most probably driving the increasing occurrence of apramycin/gentamicin cross-resistant [aac(3)-IV positive] E. coli in diseased pigs and healthy finishers at slaughter. [1] Apramycin inhibits A. pleuropneumoniae FMV 87-682 with MIC50 of 8 mg/L. Apramycin (1/4 MIC) in the medium decreases the rate of growth of the bacterial strains tested, and causes the postantibiotic effect (PAE) up to 5 hours. Apramycin significantly reduces the haemolytic activity of A. pleuropneumoniae and affects the capsular material production of this isolate and of one isolate of P. multocida (type A). [2] Apramycin induces translation errors, as assayed by incorporation of leucine, isoleucine and serine, although this effect occurs only to a limited extent, in cell-free systems from Escherichia coli programmed with poly(U). Apramycin inhibits the translocation step of protein synthesis both in vivo, in protoplasts of Bacillus megaterium, and in vitro, in cell-free systems from E. coli. [3]
In Vivo
In vivo Apramycin (225 mg/L) totally suppresses mortality and significantly reduces Salmonella excretion in comparison with non-treated chicks. [4]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05590728 Recruiting Bacterial Infection National Institute of Allergy and Infectious Diseases (NIAID) June 16 2023 Phase 1

Chemical lnformation & Solubility

Molecular Weight 637.66 Formula

C21H41N5O11.xH2SO4
CAS No. 65710-07-8 SDF Download Apramycin Sulfate SDF
Smiles CNC1C(C2C(CC(C(O2)OC3C(CC(C(C3O)O)N)N)N)OC1OC4C(C(C(C(O4)CO)N)O)O)O.OS(=O)(=O)O
Storage (From the date of receipt)

In vitro
Batch:

Water : 100 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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