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Auxinole

Auxinole is a potent auxin antagonist that binds to TIR1 and blocks the formation of the TIR1-IAA-Aux/IAA complex. Auxinole is an OsTIR1 inhibitor that suppresses leaky degradation of degron-fused proteins.

Auxinole Chemical Structure

Auxinole Chemical Structure

CAS: 86445-22-9

Purity & Quality Control

Batch: S696001 DMSO] 64 mg/mL] false] Ethanol] 5 mg/mL] false] Water] Insoluble] false Purity: 99.91%
99.91

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Biological Activity

Description Auxinole is a potent auxin antagonist that binds to TIR1 and blocks the formation of the TIR1-IAA-Aux/IAA complex. Auxinole is an OsTIR1 inhibitor that suppresses leaky degradation of degron-fused proteins.
In vitro
In vitro

Auxinole causes reduction in IAA-triggered depolarization in root hair cells. Auxinole (20 µM) also represses the transient increase in [Ca2+]cyt completely, and blocks Ca2+ response.[4]
Cell Research Cell lines Root epidermal cells of Arabidopsis thaliana
Concentrations 20 µM
Incubation Time 1-10 min
Method

Sterile grown seedlings are exposed to standard bath solution (0.1 mM KCl, 1 mM CaCl2, 5 mM MES/BTP pH 5.5; in the case of La3+-containing bath solutions ionic strength is kept constant and the sum of La3+ and Ca2+ concentrations is 1 mM). The response of the membrane potential of root epidermal cells to apoplastic and cytosolic auxin application is measured by sharp single- and double-barreled microelectrodes impaled through the tip of bulging root-hair cells. Ion fluxes across the plasma membrane of root epidermal cells are monitored using non-invasive ion-selective scanning microelectrodes.

Chemical lnformation & Solubility

Molecular Weight 321.37 Formula

C20H19NO3
CAS No. 86445-22-9 SDF --
Smiles CC1=CC(=C(C=C1)C(=O)CC(C(O)=O)C2=C[NH]C3=C2C=CC=C3)C
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 64 mg/mL ( (199.14 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 5 mg/mL

Water : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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