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Chlorzoxazone

Name: Chlorzoxazone
Brand: Selleck Chemicals
SKU: S4155

Synonyms: Chlorzoxazon,Paraflex

Catalog No.S4155 Purity: 99.8%

Chlorzoxazone(Chlorzoxazon,Paraflex) is a muscle-relaxing drug,and a probe for human liver cytochrome P-450IIE1.

Chlorzoxazone Chemical Structure

CAS: 95-25-0

Purity & Quality Control

Batch: S415501 DMSO] 34 mg/mL] false] Ethanol] 34 mg/mL] false] Water] Insoluble] false Purity: 99.8%

99.8

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Biological Activity

Description	Chlorzoxazone(Chlorzoxazon,Paraflex) is a muscle-relaxing drug,and a probe for human liver cytochrome P-450IIE1.

In vitro
In vitro	Chlorzoxazone is well documented to be metabolized mainly to 6-hydroxychlorzoxazone physically, which is catalyzed primarily by CYP2E1. Other human hepatic P450 enzymes, namely CYP1A1, CYP1A2 and CYP3A4 may also contribute to Chlorzoxazone metabolism. Chlorzoxazone has been used as a probe for CYP2E1 activity in microsomes from various sources (e.g., liver, kidney, lung cells) as well as directly in cell cultures. ^[1] Chlorzoxazone is a centrally acting skeletal muscle relaxant, which acts on the spinal cord by depressing reflexes. Chlorzoxazone is able to activate rat brain SK2 channel currents in cells. The maximal response to Chlorzoxazone is achieved at 1 mM, which is 44% ( 62%) of the response to 1 mM 1-EBIO. ^[2]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT02654236	Completed	Alcohol Consumption	Suthat Liangpunsakul\|National Institute on Alcohol Abuse and Alcoholism (NIAAA)\|Intercept Pharmaceuticals\|Indiana University	April 2016	Not Applicable
NCT00138684	Completed	Insulin Resistance\|Iron Overload	Rennes University Hospital\|Ministry of Health France	September 2003	Phase 2

References

Chemical lnformation & Solubility

Molecular Weight	169.57	Formula	C₇H₄ClNO₂
CAS No.	95-25-0	SDF	Download Chlorzoxazone SDF
Smiles	C1=CC2=C(C=C1Cl)NC(=O)O2
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 34 mg/mL ( (200.5 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 34 mg/mL

Water : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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