Home Neuronal Signaling AChR agonist - Dopamine Receptor antagonist CNO (Clozapine N-oxide) For research use only.

CNO (Clozapine N-oxide)

CNO (Clozapine N-oxide) is a metabolite of Clozapine (GLXC-06516) and an agonist of human muscarinic designer receptors (Designer Receptors Exclusively Activated by Designer Drugs, DREADDs). Clozapine is a potent dopamine (DA) antagonist and a selective muscarinic M4 receptor agonist.Solutions are unstable and should be fresh-prepared.

CNO (Clozapine N-oxide) Chemical Structure

CNO (Clozapine N-oxide) Chemical Structure

CAS: 34233-69-7

Selleck's CNO (Clozapine N-oxide) has been cited by 5 publications

Purity & Quality Control

Batch: Purity: 99.9%
99.9

Products often used together with CNO (Clozapine N-oxide)

JHU37152

Clozapine N-oxide exhibits lower in vivo DREADD potency, while JHU37152 exhibits high in vivo DREADD potency.

Bonaventura J, et al. Nat Commun. 2019 Oct 11;10(1):4627.

JHU37160

JHU37160 exhibits higher blood-brain barrier penetrance and DREADD selectivity in vivo than Clozapine N-oxide.

Savage JV, et al. Behav Brain Res. 2023 Aug 24;452:114553.

Deschloroclozapine

Clozapine N-oxide and Deschloroclozapine activate the DREADD to suppress neuronal activity in rhesus monkeys.

Eldridge MA, et al. Curr Res Neurobiol. 2023 Mar 22;4:100085.

DREADD agonist 21

Clozapine N-oxide and DREADD agonist 21 repeated administration contributes to an unusual organization of the gut microbiota in adult mice.

Guo W, et al. Brain Res Bull. 2021 Aug;173:66-73.

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Biological Activity

Description CNO (Clozapine N-oxide) is a metabolite of Clozapine (GLXC-06516) and an agonist of human muscarinic designer receptors (Designer Receptors Exclusively Activated by Designer Drugs, DREADDs). Clozapine is a potent dopamine (DA) antagonist and a selective muscarinic M4 receptor agonist.Solutions are unstable and should be fresh-prepared.
Targets
DREADD [1] Dopamine receptor [2] muscarinic M4 receptor [1]
In vitro
In vitro

CNO is a synthetic small molecule actuator for muscarinic acetylcholine DREADDs.
In Vivo
In vivo

Clozapine N-oxide (CNO), an agonist of human muscarinic designer receptor (DREADDs), is converted to clozapine and exerts clozapine-like behavioral effects in both mice and rats further emphasizes the need for appropriate control groups in studies employing DREADDs.[4]
Animal Research Animal Models standard laboratory rats and mice
Dosages 1.0, 3.2, 10.0 mg/kg for rats; 1.25, 2.5, 5.0, 10.0, 20.0 mg/kg for mice
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00372242 Completed Mild and Moderate Malnourished Children International Centre for Diarrhoeal Disease Research Bangladesh December 2000 Not Applicable

Chemical lnformation & Solubility

Molecular Weight 342.82 Formula

C18H19ClN4O
CAS No. 34233-69-7 SDF --
Smiles C[N+]1([O-])CCN(CC1)C2=NC3=C(NC4=CC=CC=C24)C=CC(=C3)Cl
Storage (From the date of receipt) 3 years -20°C powder Solutions are unstable. Prepare fresh or purchase small, pre-packaged sizes. Repackage upon receipt.

In vitro
Batch:

DMSO : 68 mg/mL ( (198.35 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 68 mg/mL

Ethanol : 8 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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