Home Microbiology Antineoplastic and Immunosuppressive Antibiotics inhibitor - Bacterial chemical - MMP inhibitor - Antibiotics chemical Doxycycline For research use only.

Doxycycline

Synonyms: Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum

Doxycycline (Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum) is an antibiotic that is used in the treatment of a number of types of infections caused by bacteria and protozoa. Doxycycline is also a nonspecific matrix metalloproteinase (MMP) inhibitor.

Doxycycline Chemical Structure

Doxycycline Chemical Structure

CAS: 564-25-0

Selleck's Doxycycline has been cited by 35 publications

Purity & Quality Control

Batch: Purity: >97%
97

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Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Staphylococcus aureus 2 planktonic cells Bactericidal assay 24 hrs Bactericidal activity against methicillin-resistant Staphylococcus aureus 2 planktonic cells after 24 hrs by calgary biofilm device method, MBC=2μM. 29638121
THP1 Function assay Selectivity index, ratio of IC50 for human THP1 cells to IC50 for Plasmodium falciparum, IC50=3.1μM. 19748781
K562 Cytotoxicity assay 72 hrs Cytotoxicity against human K562 cells after 72 hrs by flow cytometry, IC50=15μM. 19482476
K562 Cytotoxicity assay 72 hrs Cytotoxicity against human K562 cells after 72 hrs by flow cytometry, IC50=15μM. 19926173
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay, IC50=20μM. 19482476
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay, IC50=20μM. 19926173
THP1 Cytotoxicity assay 72 hrs Cytotoxicity against human THP1 cells after 72 hrs by propidium iodide staining-based flow cytometry, IC50=20μM. 19748781
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay, CC50=20μM. 21741131
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay, CC50=20μM. 22889559
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay, CC50=20μM. 21852132
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay, CC50=20μM. 24946216
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells assessed as cell viability after 72 hrs by MTT assay, CC50=20μM. 25791675
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells incubated for 72 hrs by MTT assay, CC50=20μM. 25282267
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells measured after 72 hrs by MTT assay, CC50=20μM. 27155463
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay, CC50=20μM. 27654395
SW1353 Function assay 50 uM Inhibition of IL-1-beta-induced MMP13 production in human SW1353 cells at 50 uM 17267227
K-12 BW25113 Bactericidal assay 24 hrs Bactericidal activity against yafQ gene-deficient Escherichia coli K-12 BW25113 biofilm assessed as log reduction of viable cells after 24 hrs 19307375
K-12 BW25113 Bactericidal assay 24 hrs Bactericidal activity against Escherichia coli K-12 BW25113 biofilm harboring pCA24N ptac::yafQ plasmid assessed as log reduction of viable cells after 24 hrs pretreated with 5 uM of IPTG for 4 hrs 19307375
Neuro2a Function assay 1 uM Decrease in 7-DHC levels in Dhcr7-deficient mouse Neuro2a cells at 1 uM by LC-MS/GC-MS analysis 26789657
vascular endothelial cells Antibacterial assay 25 ug/ml 72 hrs Antibacterial activity against Rickettsia prowazekii str. Breinl infected in CD rat primary pulmonary vascular endothelial cells assessed as bacterial shape change at 25 ug/ml measured 72 hrs post infection by Hoechst 33258/phalloidin staining based fluor 28089350
A673 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells 29435139
BT-12 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-12 cells 29435139
Hep2 Anti-Chlamydial assay 1 ug/ml 8 hrs Anti-Chlamydial activity against Chlamydia trachomatis Serovar LGV-L2 infected in Hep2 cells assessed as reduction in size and number of chlamydial inclusion at 1 ug/ml treated at 8 hrs post-infection and 24 hrs later re-infecting fresh Hep2 cells monolay 32227948
skeletal myoblast cells Cytotoxicity assay DNDI: Cytotoxicity in Vitro, 72 hour, in rat skeletal myoblast cells, IC50=14.49μM. ChEMBL
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Biological Activity

Description Doxycycline (Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum) is an antibiotic that is used in the treatment of a number of types of infections caused by bacteria and protozoa. Doxycycline is also a nonspecific matrix metalloproteinase (MMP) inhibitor.
In vitro
In vitro

100 ng/mL-5 µg/mL Dox can significantly alter the metabolic profile of the cell, as well as reduce the proliferative rate, though the effect size depends upon the particular cell line used[1].

Doxycycline arrests cells in G1-S phase of the cell cycle in PANC-1 cells and induces the expression of p53 and its downstream target p21. It down-regulates antiapoptotic genes and induces proapoptotic genes. Doxycycline also induces apoptosis through a Fas/Fas-ligand dependent pathway in Jurkat T lymphocytes[2].
Cell Research Cell lines U87 astrocytoma cells, lung cancer cell lines H157 and H1437, LNCaP prostate cancer and HeLa cervical cancer cell lines
Concentrations 100 ng/mL, 1 µg/mL
Incubation Time 24, 72, and 96 hours
Method

Cells are seeded in 6 well plates at 50,000 cells per well and treated with Dox (100 ng/mL, 1 µg/mL, or vehicle control). Cells are counted 24, 72, and 96 hours after plating using a particle counter.
Experimental Result Images Methods Biomarkers Images PMID
Western blot AKT / BCL6 / Cyclin E / HDAC2 / HDAC3 / NEMO / TYK2 / RIPK1 HSP70 / HSP90 pATM / ATM 26142707
Immunofluorescence E-cadherin / Vimentin 29285218
In Vivo
In vivo

Doxycycline successfully reduces tumor growth in vivo (inhibited pancreatic cancer cell growth)[2].
Animal Research Animal Models Male nu/nu mice
Dosages 35 mg/pellet
Administration s.c.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05972772 Not yet recruiting Infectious Disease|Therapeutics Lao-Oxford-Mahosot Hospital Wellcome Trust Research Unit|Mahidol Oxford Tropical Medicine Research Unit February 1 2024 Phase 2|Phase 3
NCT06007534 Not yet recruiting Post-exposure Prophylaxis|Sexually Transmitted Diseases|Doxycycline Assistance Publique - Hôpitaux de Paris September 2023 Not Applicable
NCT04762134 Recruiting Bacterial Sexually Transmitted Diseases Jonathan Troy Grennan|Canadian Institutes of Health Research (CIHR)|British Columbia Centre for Disease Control June 2 2023 Phase 4
NCT05853120 Recruiting Sexually Transmitted Diseases Emory University|Centers for Disease Control and Prevention May 31 2023 Phase 4
NCT05382208 Recruiting Emphysema|HIV Weill Medical College of Cornell University|National Heart Lung and Blood Institute (NHLBI)|University of California Los Angeles|University of Iowa|University of Michigan August 22 2022 Phase 2
NCT05492019 Recruiting Parkinson Disease Bangabandhu Sheikh Mujib Medical University Dhaka Bangladesh July 1 2022 Phase 2

Chemical lnformation & Solubility

Molecular Weight 444.43 Formula

C22H24N2O8
CAS No. 564-25-0 SDF Download Doxycycline SDF
Smiles CC1C2C(C3C(C(=O)C(=C(C3(C(=O)C2=C(C4=C1C=CC=C4O)O)O)O)C(=O)N)N(C)C)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 89 mg/mL ( (200.25 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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