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Ethambutol 2HCl

Name: Ethambutol 2HCl
Brand: Selleck Chemicals
SKU: S4004
Rating: 5 (2 reviews)

Synonyms: CL40881

Catalog No.S4004 Purity: 100%

Ethambutol 2HCl (CL40881) is a bacteriostatic antimycobacterial agent, which obstructs the formation of cell wall by inhibiting arabinosyl transferases.

Ethambutol 2HCl Chemical Structure

CAS: 1070-11-7

Selleck's Ethambutol 2HCl has been cited by 2 publications

Purity & Quality Control

Batch: S400401 DMSO] 56 mg/mL] false] Water] 56 mg/mL] false] Ethanol] Insoluble] false Purity: 100%

100

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Biological Activity

Description	Ethambutol 2HCl (CL40881) is a bacteriostatic antimycobacterial agent, which obstructs the formation of cell wall by inhibiting arabinosyl transferases.

In vitro
In vitro	Ethambutol directly affects two polymers, arabinogalactan (AG) and lipoarabinomannan (LAM) in Mycobacterium smegmatis. In M. smegmatis, Ethambutol inhibits synthesis of arabinan completely and inhibits AG synthesis most likely as a consequence of this; more than 50% of the cell arabinan is released from the bacteria following Ethambutol treatment, whereas no galactan is released. Ethambutol main targets against embB gene product in M. avium. Ethambutol induces 60% changes in the embB gene in M. tuberculosis resistant mutants. ^[1] Ethambutol is effective against actively growing microorganisms of the genus Mycobacterium, including M. tuberculosis. Nearly all strains of M. tuberculosis and M. kansasii as well as a number of strains of the M. aviumcomplex (MAC) are sensitive to Ethambutol. ^[1] Ethambutol is potency against M. tuberculosis (H37Rv) with MIC of 0.5 μg/mL in vitro. ^[2] Ethambutol is efficient on treatment of mycobacterial-infected macrophages. When M. tuberculosis infected macrophages are treated with 6 μg/mL Ethambutol, the log CFUs following treatment for 3 days is 4.17, while value in control group is 4.8. The MICs for M. avium (MTCC 1723) and M. smegmatis (MTCC 6) are 15 μg/mL and 0.18 μg/mL, respectively. ^[3]

In Vivo
In vivo	Ethambutol is efficient in animal model. 100 mg/kg Ethambutol given orally 15 days post i.v. infection 1 ×/week for 5 weeks, induces a lower log CFU compared with untreatment (4.59 vs 5.07). ^[3]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT05840809	Recruiting	Tuberculosis Active\|Breastfeeding\|Tuberculosis	University of Liverpool\|Infectious Diseases Institute Makerere University College of Health Sciences	January 20 2022	--
NCT04972903	Unknown status	Tuberculosis\|Pulmonary	Institut National de la Santé Et de la Recherche Médicale France\|National Agency for Research on AIDS and Viral Hepatitis (ANRS)	August 2021	--
NCT02114684	Completed	Recurrent Tuberculosis	Centre for the AIDS Programme of Research in South Africa	November 2013	Phase 1\|Phase 2
NCT02496572	Unknown status	Multidrug Resistant Tuberculosis	Medecins Sans Frontieres Netherlands\|Ministry of Health Republic of Uzbekistan\|Imperial College London	September 2013	--

References

Chemical lnformation & Solubility

Molecular Weight	277.23	Formula	C₁₀H₂₄N₂O₂.2HCl
CAS No.	1070-11-7	SDF	--
Smiles	CCC(CO)NCCNC(CC)CO.Cl.Cl
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 56 mg/mL ( (201.99 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 56 mg/mL

Ethanol : Insoluble

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In vivo
Batch:

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In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

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Handling Instructions

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