Home PI3K/Akt/mTOR mTOR activator - Autophagy inhibitor MHY1485 For research use only.

MHY1485

MHY1485 is a potent, and cell-permeable mTOR activator, and also potently inhibits autophagy.

MHY1485 Chemical Structure

MHY1485 Chemical Structure

CAS: 326914-06-1

Selleck's MHY1485 has been cited by 76 publications

Purity & Quality Control

Batch: Purity: 99.97%
99.97

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Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HT-29  Function assay 10 nM, 100 nM, 1 μM, 10 μM 48 h significantly increased the expression of miR-212 in a dose-dependent manner 30021100
RSC96 Function assay 10 μM 1 day or 2 days MHY1485 increased phospho-mTOR (Ser 2448), phosphoS6K1 (Thr 389) and NGF expression in RSC96 cells. 30229399
H9C2 Function assay 5 μM MHY1485 abrogated the effects of Tanshinone IIA on LC3 30798134
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Biological Activity

Description MHY1485 is a potent, and cell-permeable mTOR activator, and also potently inhibits autophagy.
Targets
mTOR [1]
In vitro
In vitro

MHY1485 suppresses the basal autophagic flux. MHY1485 causes the accumulation of LC3II and enlargement of the autophagosomes in a dose- and time- dependent manner.[1]

MHY1485 increases phospho-mTOR levels and phosphorylation of downstream S6K1 and rpS6 proteins without affecting total mTOR content, total S6K1 and rpS6 levels. Short-term treatment of ovaries with MHY1485 followed by allo-transplantation promoted secondary follicle growth. Treatment with MHY1485 and subsequent grafting allowed the derivation of mature oocytes and healthy offspring.[2]
Kinase Assay Activation of mTOR
Western blot analysis is performed to detect the change of total protein level and levels of phosphorylated forms of mTOR and 4E-BP1 reflecting the activity of mTOR. Ac2F cells are treated with MHY1485 of different concentrations and rapamycin 5 mM as a positive control for 1 h. Cells are washed with cold PBS and harvested. Cell lysates are prepared using RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, 1% NP-40, 1 mM DTT, 0.1 mM NaF, 1 mM PMSF, 1 mg/mL pepstatin, 1 mg/mL leupeptin, and 1 mg/mL aprotinin). Protein concentration is determined by the bicinchoninic acid (BCA) method. Equal amounts of protein are separated on 10-12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels. The gels are subsequently transferred onto a polyvinylidene difluoride membrane by electroblotting for 2 h at 60-75 V. The membranes are blocked in a 5% nonfat milk solution in Tris-buffered saline (TBS) with 0.5% Tween-20, and incubated with primary antibodies. Pre-stained protein markers are used for molecular-weight determination.
Cell Research Cell lines TU177 cells
Concentrations 10 µM
Incubation Time 24 h
Method

Cells were treated with indicated concentration of drug for 24 h.
Experimental Result Images Methods Biomarkers Images PMID
Western blot p-Nrf2 / Nrf2 p-mTOR / mTOR / p-AKT / AKT / p-S6K1 / S6K1 28061443
In Vivo
In vivo

MHY1485 is a potent, and cell-permeable mTORC1 agonist.

Chemical lnformation & Solubility

Molecular Weight 387.39 Formula

C17H21N7O4
CAS No. 326914-06-1 SDF Download MHY1485 SDF
Smiles C1COCCN1C2=NC(=NC(=N2)NC3=CC=C(C=C3)[N+](=O)[O-])N4CCOCC4
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 10 mg/mL ( (25.81 mM)； Warmed with 50°C water bath; Ultrasonicated; Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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