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Pregnenolone

Name: Pregnenolone
Brand: Selleck Chemicals
SKU: S1914
Rating: 5 (3 reviews)

Synonyms: 3β-Hydroxy-5-pregnen-20-one

Catalog No.S1914 Purity: 100%

Pregnenolone(3β-Hydroxy-5-pregnen-20-one) is an endogenous steroid hormone, used in the treatment of fatigue, Alzheimer’s disease, trauma and injuries.

Pregnenolone Chemical Structure

CAS: 145-13-1

Selleck's Pregnenolone has been cited by 3 publications

Purity & Quality Control

Batch: Purity: 100%

100

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Biological Activity

Description

Pregnenolone(3β-Hydroxy-5-pregnen-20-one) is an endogenous steroid hormone, used in the treatment of fatigue, Alzheimer’s disease, trauma and injuries.

Targets

progestogen Receptor ^[1]

In vitro
In vitro	Pregnenolone induces a large, dose-related increase of both the rate and extent of MAP2-induced tubulin assembly, whereas progesterone, inactive per se, counteracted the stimulatory effect of Pregnenolone. Pregnenolone-increased assembly of microtubules produces a completely normal structure. ^[1] Pregnenolone preserves microtubule abundance and promotes cell movement during epiboly. ^[2] Pregnenolone results in dramatic reduction in GR nuclear localization in mouse hippocampal cell line (HT-22). Pregnenolone has neuroprotective effects against both glutamate and amyloid beta protein neuropathology. ^[3]

In Vivo
In vivo	Pregnenolone sustains its proliferative activity in vivo and stimulates the growth of LNCaP-tumor xenografts in intact male SCID mice as well as in castrated animals. Pregnenolone is shown to activate transcription through the LNCaP androgen receptor (AR), but not the wild-type AR. ^[4] Pregnenolone results in a significant decrease in the accumulation of astrocytes in the proximity of the wound and in a decreased bromodeoxyuridine incorporation in reactive astrocytes of rats. ^[5] Pregnenolone administration results in elevations in downstream neurosteroids such as allopregnanolone, a molecule with neuroprotective effects that also increases neurogenesis, decreases apoptosis and inflammation, modulates the hypothalamic-pituitary-adrenal axis, and markedly increases GABA(A) receptor responses. Pregnenolone administration elevates pregnenolone sulfate, a neurosteroid that positively modulates NMDA receptors. ^[6]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT05781009	Not yet recruiting	Alcohol Use Disorder	Yale University\|National Institute on Alcohol Abuse and Alcoholism (NIAAA)	January 15 2024	Phase 2
NCT05903716	Recruiting	Acne Vulgaris	Marmara University	September 15 2022	--
NCT04791124	Unknown status	Down Syndrome	Parc de Salut Mar\|Aelis Farma\|Starlab	January 11 2021	--
NCT03801629	Completed	Drug Effect	Yale University	September 12 2019	Phase 1
NCT03953612	Recruiting	Cocaine-Related Disorders	Yale University	March 12 2019	Early Phase 1

References

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Chemical lnformation & Solubility

Molecular Weight	316.48	Formula	C₂₁H₃₂O₂
CAS No.	145-13-1	SDF	Download Pregnenolone SDF
Smiles	CC(=O)C1CCC2C1(CCC3C2CC=C4C3(CCC(C4)O)C)C
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 22 mg/mL ( (69.51 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 22 mg/mL

Water : Insoluble

Molecular Weight Calculator

In vivo
Batch:

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In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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