Home Stem Cells & Wnt Wnt/beta-catenin agonist SKL2001 For research use only.

SKL2001

SKL2001 is a novel agonist of the Wnt/β-catenin pathway. It disrupts the Axin/β-catenin interaction.

SKL2001 Chemical Structure

SKL2001 Chemical Structure

CAS: 909089-13-0

Selleck's SKL2001 has been cited by 45 publications

Purity & Quality Control

Batch: Purity: 99.49%
99.49

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Biological Activity

Description SKL2001 is a novel agonist of the Wnt/β-catenin pathway. It disrupts the Axin/β-catenin interaction.
Targets
Wnt/β-catenin [1]
(Cell-free assay)
In vitro
In vitro SKL2001 upregulated β-catenin responsive transcription by increasing the intracellular β-catenin protein level and inhibited the phosphorylation of β-catenin at residues Ser33/37/Thr41 and Ser45, which would mark it for proteasomal degradation, without affecting CK1 and GSK-3β enzyme activities. SKL2001 disrupted the Axin/β-catenin interaction, which is a critical step for CK1- and GSK-3β-mediated phosphorylation of β-catenin at Ser33/37/Thr41 and Ser45. The treatment of mesenchymal stem cells with SKL2001 promoted osteoblastogenesis and suppressed adipocyte differentiation, both of which were accompanied by the activation of Wnt/β-catenin pathway. SKL2001 did not affect either NF-κB or p53 reporter activity and inhibits β-catenin phosphorylation without affecting GSK-3β activity[1].
Cell Research Cell lines HEK293 reporter and control cell lines
Concentrations 20 μM
Incubation Time 15 h
Method The HEK293 reporter and control cell lines were established. The HEK293 reporter cells were inoculated into 384-well plates at 10 000 cells per well and grown for 24 h. Next, each compound in the chemical library (∼270 000) was added to at a final concentration of 20 μM. After 15 h, the plates were assayed for firefly luciferase activity.
Experimental Result Images Methods Biomarkers Images PMID
Western blot β-catenin PPARγ / C/EBPα 21826110
Immunofluorescence β-catenin 21826110

Chemical lnformation & Solubility

Molecular Weight 286.29 Formula

C14H14N4O3
CAS No. 909089-13-0 SDF Download SKL2001 SDF
Smiles C1=COC(=C1)C2=CC(=NO2)C(=O)NCCCN3C=CN=C3
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 57 mg/mL ( (199.09 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 57 mg/mL

Water : Insoluble


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