Home Endocrinology & Hormones Androgen Receptor antagonist Spironolactone For research use only.

Spironolactone

Spironolactone is a potent antagonist of the androgen receptor with IC50 of 77 nM.

Spironolactone Chemical Structure

Spironolactone Chemical Structure

CAS: 52-01-7

Selleck's Spironolactone has been cited by 11 Publications

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Purity & Quality Control

Batch: Purity: 99.80%
99.80

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Biological Activity

Description Spironolactone is a potent antagonist of the androgen receptor with IC50 of 77 nM.
Targets
Androgen Receptor [1]
77 nM
In vitro
In vitro Spironolactone is a strong AR antagonist (IC50 ~ 77 nM), a weak GR antagonist (IC50 ~ 2.4 μM), and a weak PR agonist (EC50 ~ 740 nM). [1] Spironolactone inhibits androstanolone binding in rat prostate nuclei and androstanolone specific binding in rat prostate cytosol. [2]
In Vivo
In vivo Spironolactone (1 mg/day) exerts anti-androgenic activity in rats. A single pretreatment of spironolactone (1 mg/rat) inhibits specific and saturable uptake of hormone into prostate induced by tracer dose administration of [3H]testosterone. [2]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06021860 Not yet recruiting Edema Due to Heart Failure or Cirrhosis CMP Development LLC June 2024 Phase 4
NCT05807139 Recruiting Alcohol Use Disorder National Institute on Drug Abuse (NIDA)|National Institutes of Health Clinical Center (CC) January 12 2024 Phase 1
NCT05092984 Recruiting Rheumatoid Arthritis University Hospital Strasbourg France June 22 2022 Phase 3
NCT04723862 Recruiting Hyperandrogenism|Polycystic Ovary Syndrome|Puberty University of Virginia|Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) November 12 2021 Early Phase 1

Chemical lnformation & Solubility

Molecular Weight 416.57 Formula

C24H32O4S
CAS No. 52-01-7 SDF Download Spironolactone SDF
Smiles CC(=O)SC1CC2=CC(=O)CCC2(C3C1C4CCC5(C4(CC3)C)CCC(=O)O5)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 83 mg/mL ( (199.24 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 27 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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