Home Microbiology Bacterial inhibitor - Antibiotics inhibitor - Antineoplastic and Immunosuppressive Antibiotics chemical Tetracycline For research use only.

Tetracycline

Synonyms: TC

Tetracycline (TC) is a broad-spectrum antibiotic that exhibits activity against a wide range of microorganisms including gram-positive and gram-negative bacteria, chlamydiae, mycoplasmas, rickettsiae, and protozoan parasites.

Tetracycline Chemical Structure

Tetracycline Chemical Structure

CAS: 60-54-8

Selleck's Tetracycline has been cited by 1 publication

Purity & Quality Control

Batch: Purity: 99.86%
99.86

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Biological Activity

Description Tetracycline (TC) is a broad-spectrum antibiotic that exhibits activity against a wide range of microorganisms including gram-positive and gram-negative bacteria, chlamydiae, mycoplasmas, rickettsiae, and protozoan parasites.
In vitro
In vitro

Tetracycline-induced CK2 expression significantly increases the non–small cell lung cancer (NSCLC) invasion in cells expressing BRMS1 wild-type, but not the S30A mutant.[2]
Cell Research Cell lines H157 BRMS1 knockdown (KD) cells, H157 stable cells
Concentrations 1 µg/mL
Incubation Time 48 h
Method

H157 BRMS1 knockdown (KD) cells were cotransfected with pcDNA3-luciferase and Tet-on CK2α'. Stable clones were selected by Geneticin (400 µg/mL), Blasticidin (5 µg/mL), and Zeocin (150 µg/mL). Myc/His(6)- CK2α' expression was confirmed by immunoblot after treatment with TCN (1 µg/mL) for 48 h. Then, the H157 BRMS1KD/luciferase/Tet-on CK2α' cells were infected with pBabe-shRNA–resistant FLAG-BRMS1 wild-type, S30A mutant, or empty vector and selected with puromycin (1 µg/mL). Flag-BRMS1 expression was confirmed by Western blot. H157 stable cells were pretreated with or without TCN (1 µg/mL) for 48 h. the inserts were coated with type IV collagen at a concentration of 0.25 mg/ml. The bottom portion of the wells included 1% FBS-media. Cells were allowed to migrate for 6 hours or invade for 22 hours. At the specified time point, inserts were fixed and stained with crystal violet.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06159660 Not yet recruiting Lung Neoplasm University of Sydney|National Health and Medical Research Council Australia|Northern Sydney and Central Coast Area Health Service March 1 2024 --
NCT06005610 Not yet recruiting HIV I Infection National Institute of Allergy and Infectious Diseases (NIAID) January 2 2024 Phase 2

Chemical lnformation & Solubility

Molecular Weight 444.43 Formula

C22H24N2O8
CAS No. 60-54-8 SDF --
Smiles CN(C)C1C2CC3C(=C(O)C4=C(C=CC=C4O)C3(C)O)C(=O)C2(O)C(=C(C(N)=O)C1=O)O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 89 mg/mL ( (200.25 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 22 mg/mL

Water : Insoluble


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In vivo
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