Tocilizumab (anti-IL-6R)

Synonyms: MRA

Catalog No.A2012 Purity: 99%

Tocilizumab (anti-IL-6R) is a humanized monoclonal antibody that binds to the interleukin-6 receptor, MW: 148 KD.

Tocilizumab (anti-IL-6R) Chemical Structure

Click to purchase the isotype control of Tocilizumab (anti-IL-6R)

Selleck's Tocilizumab (anti-IL-6R) has been cited by 42 publications

Purity & Quality Control

Batch: Purity: 99% Protein concentration: 14.29mg/ml Endotoxin Level: ＜1EU/mg

Choose Selective IL Receptor Inhibitors

Biological Activity

Description

Tocilizumab (anti-IL-6R) is a humanized monoclonal antibody that binds to the interleukin-6 receptor, MW: 148 KD.

Features

Human IgG1

Targets

IL-6R ^[1]

In Vitro
In vitro	After adding tocilizumab at different concentrations into HMO6 and CHME5 cells stimulated by F3-CM or with IL6, the expression levels of anti-inflammatory M2 markers (CD206 and Arg1) are decreased, as well as the levels of both phosphorylated JAK2 and STAT3.^[1]
Cell Research:	HMO6 and CHME5 cells with control media or F3-CM alone or F3-CM were treated with Tocilizumab (2.5 μg/ml for 24 h) or with IL6 (200 ng/ml for 24 h) alone or IL6-supplemented with Tocilizumab. Western blot analysis of p-JAK2, JAK2, p-STAT3, and STAT3 in HMO6 and CHME5 cells were conducted.

In Vitro

In vitro

After adding tocilizumab at different concentrations into HMO6 and CHME5 cells stimulated by F3-CM or with IL6, the expression levels of anti-inflammatory M2 markers (CD206 and Arg1) are decreased, as well as the levels of both phosphorylated JAK2 and STAT3.^[1]

Cell Research:

HMO6 and CHME5 cells with control media or F3-CM alone or F3-CM were treated with Tocilizumab (2.5 μg/ml for 24 h) or with IL6 (200 ng/ml for 24 h) alone or IL6-supplemented with Tocilizumab. Western blot analysis of p-JAK2, JAK2, p-STAT3, and STAT3 in HMO6 and CHME5 cells were conducted.

In Vivo
In vivo	Tocilizumab can block JAK2/STAT3 activation and impede brain metastasis (BM) of A549-F3 cells in vivo.^[1]
Animal Research	Animal Models: Nude mice of brain metastasis models Dosages: 5 mg/kg Administration: i.p. Reference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864012/

References
[1] Jin Y, et al. Signal Transduct Target Ther. 2022 Feb 23;7(1):52.

References

[1] Jin Y, et al. Signal Transduct Target Ther. 2022 Feb 23;7(1):52.

Product Details

CAS No.	‎375823-41-9
Isotype	Human IgG1
Source	CHO cells
Formulation	PBS buffer, pH 7.2
Storage (From the date of receipt)	Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

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Frequently Asked Questions

Question 1:
How to store the antibody?

Answer:
Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.