Home DNA Damage/DNA Repair Topoisomerase inhibitor Topotecan For research use only.

Topotecan

Synonyms: NSC609699,Nogitecan,SKFS 104864A

Topotecan(NSC609699,Nogitecan,SKFS 104864A) is an antineoplastic agent used to treat ovarian cancer that works by inhibiting DNA topoisomerases.

Topotecan Chemical Structure

Topotecan Chemical Structure

CAS: 123948-87-8

Selleck's Topotecan has been cited by 25 publications

Purity & Quality Control

Batch: Purity: 99.94%
99.94

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Biological Activity

Description Topotecan(NSC609699,Nogitecan,SKFS 104864A) is an antineoplastic agent used to treat ovarian cancer that works by inhibiting DNA topoisomerases.
Targets
Topo I [1]
In vitro
In vitro

Topotecan exhibits inhibition of outgrowth of the highly invasive and migratory leader cells (LC) population, however, topotecan enrichs the LC subset 20% above the untreated control level.[2]
Cell Research Cell lines LC-T2A-GFP lines
Concentrations 1 μM
Incubation Time 72 h
Method

LC-T2A-GFP lines are seeded at 1200 cells per well of a 384-well plate, adhered overnight, and treated with chemotherapeutics olaparib, rucaparib, paclitaxel, topotecan, carboplatin, cyclophosphamide, cisplatin, and doxorubicin at empirically determined clinically relevant doses (1 μM). Cells are maintained at 37 ℃ with 5% CO2 in treatment for 72 h. Four focal areas per replicate well were imaged using 4X magnification on a bright field.
In Vivo
In vivo

Topotecan (TPT), at least in part by inhibiting expression of Fli-1, significantly ameliorates lupus nephritis in NZBWF1 mice as effectively or better than cyclophosphamide (CYC).[3]
Animal Research Animal Models NZBWF1 female mice
Dosages 0.03 mg/kg, 0.1 mg/kg, 0.3mg/kg
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT04455139 Terminated Eye Cancer Retinoblastoma Prof. Beck Popovic Maja|University of Lausanne Hospitals November 15 2021 Phase 2
NCT05083000 Unknown status COVID-19 Respiratory Infection National University Hospital Singapore|Christian Medical College Vellore India August 16 2021 Phase 1
NCT04156347 Active not recruiting Retinoblastoma Targeted Therapy Technologies LLC June 16 2021 Phase 1
NCT04428879 Recruiting Retinoblastoma The Hospital for Sick Children June 16 2020 Phase 1

Chemical lnformation & Solubility

Molecular Weight 421.45 Formula

C23H23N3O5
CAS No. 123948-87-8 SDF --
Smiles CCC1(C2=C(COC1=O)C(=O)N3CC4=CC5=C(C=CC(=C5CN(C)C)O)N=C4C3=C2)O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 84 mg/mL ( (199.31 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : ˂1 mg/mL

Ethanol : ˂1 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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