Home Transmembrane Transporters ATPase inhibitor Acevaltrate For research use only.

Acevaltrate

Acevaltrate, an active component derived from the herbal plant Valeriana jatamansi Jones, is strikingly potent to induce GBM cell apoptosis. Acevaltrate inhibits the Na+/K+-ATPase activity in the rat kidney and brain hemispheres with IC50 of 22.8 μM and 42.3 μM, respectively.

Acevaltrate Chemical Structure

Acevaltrate Chemical Structure

CAS: 25161-41-5

Purity & Quality Control

Batch: E024401 DMSO] 96 mg/mL] false] ] ] false] ] ] false Purity: 99.89%
99.89

Other ATPase Products

Related compound libraries

Choose Selective ATPase Inhibitors

Biological Activity

Description Acevaltrate, an active component derived from the herbal plant Valeriana jatamansi Jones, is strikingly potent to induce GBM cell apoptosis. Acevaltrate inhibits the Na+/K+-ATPase activity in the rat kidney and brain hemispheres with IC50 of 22.8 μM and 42.3 μM, respectively.
Targets
Na+/K+-ATPase [1]
(in the rat kidney)		 Na+/K+-ATPase [1]
(in the rat brain hemispheres)
22.8 μM 42.3 μM
In vitro
In vitro

Acevaltrate disrupts the interaction of Otub1/c-Maf thus inhibiting Otub1 activity and leading to c-Maf polyubiquitination and subsequent degradation in proteasomes. Consistently, acevaltrate inhibits c-Maf transcriptional activity and downregulates the expression of its target genes key for myeloma growth and survival.[1]
Cell Research Cell lines MM cell lines (RPMI-8226, LP1, KMS11, OPM2, U266)
Concentrations 1, 2, 4 µM
Incubation Time 24 h
Method

RPMI-8226 cells are transfected with plasmids as indicated for 48 h. Cells are then re-plated and treated with 0.1% DMSO or acevaltrate for 24 h before being harvested for luciferase activity measurements. MM cells are treated with acevaltrate for 24 h, followed by WB or RT-PCR assays to evaluate the expression of specifc genes.
In Vivo
In vivo

Acevaltrate displays potent anti-myeloma activity by triggering myeloma cell apoptosis in vitro and impairing myeloma xenograft growth in vivo but presents no marked toxicity.[1]
Animal Research Animal Models Nude mice of myeloma xenograft model
Dosages 50 mg/kg
Administration p.o.

Chemical lnformation & Solubility

Molecular Weight 480.51 Formula

C24H32O10
CAS No. 25161-41-5 SDF --
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 96 mg/mL ( (199.78 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Acevaltrate | Acevaltrate supplier | purchase Acevaltrate | Acevaltrate cost | Acevaltrate manufacturer | order Acevaltrate | Acevaltrate distributor