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Ethinyl Estradiol

Name: Ethinyl Estradiol
Brand: Selleck Chemicals
SKU: S1625
Rating: 5 (2 reviews)

Catalog No.S1625 Purity: 99.85%

Ethinyl Estradiol is an orally bio-active estrogen used in almost all modern formulations of combined oral contraceptive pills.

Ethinyl Estradiol Chemical Structure

CAS: 57-63-6

Selleck's Ethinyl Estradiol has been cited by 2 publications

Purity & Quality Control

Batch: Purity: 99.85%

99.85

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Biological Activity

Description

Ethinyl Estradiol is an orally bio-active estrogen used in almost all modern formulations of combined oral contraceptive pills.

Targets

Estrogen receptor ^[1]

In vitro
In vitro	Ethinyl Estradiol increases respiratory chain activity in both cultured rat hepatocytesand HepG2 cells. Ethinyl estradiol is a strong promoter of hepatocarcinogenesis. ^[1] Ethinyl Estradiol enhances the transcript levels of nuclear genome- and mitochondrial genome-encoded genes and respiratory chain activity in female rat liver, and also inhibits transforming growth factor beta (TGFbeta)-induced apoptosis in cultured liver slices and hepatocytes from female rats. Ethinyl Estradiol increases the transcript levels of the mitochondrial genome-encoded genes cytochrome oxidase subunits I, II, and III in cultured female rathepatocytes. Ethinyl Estradiol significantly increases both the levels of glutathione (reduced [GSH] and oxidized [GSSG] forms) per mg protein in mitochondria and nuclei, while the percentage of total glutathione in the oxidized form is not affected. ^[2]

In Vivo
In vivo	Ethinyl Estradiol (50 mg/kg/day) increases anogenital distance and reduces pup body weight at postnatal day 2, accelerates the age at vaginal opening, reduces F1 fertility and F2 litter sizes, and induces malformations of the external genitalia (5 mg/kg) in the female Long-Evans rat. ^[3] Ethinyl Estradiol increases the number of low density lipoprotein (LDL) receptors in livers of rats, thereby producing a profound fall in plasma cholesterol levels. Ethinyl Estradiol exerts the same effect in livers of male and female rabbits and that the increase in receptor number is correlated with a 6- to 8-fold increase in the levels of receptor mRNA. ^[4]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT06039826	Active not recruiting	Overweight	Eli Lilly and Company	September 12 2023	Phase 1
NCT05671653	Terminated	Obesity	Pfizer	January 19 2023	Phase 1
NCT05360550	Completed	Pregnancy Prevention	Lupin Research Inc	July 5 2022	Phase 2
NCT05058872	Recruiting	Oral Hormonal Contraceptive Use\|Neural Activity\|Mood\|Hedonic Function	University of Colorado Denver	October 1 2021	Phase 4

References

[4] Ma PT, et al. Proc Natl Acad Sci U S A,?986, 83(3), 792-796.

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Chemical lnformation & Solubility

Molecular Weight	296.4	Formula	C₂₀H₂₄O₂
CAS No.	57-63-6	SDF	Download Ethinyl Estradiol SDF
Smiles	CC12CCC3C(C1CCC2(C#C)O)CCC4=C3C=CC(=C4)O
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 59 mg/mL ( (199.05 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 59 mg/mL

Water : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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