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Etonogestrel

Name: Etonogestrel
Brand: Selleck Chemicals
SKU: S4673
Rating: 5 (2 reviews)

Synonyms: Implanon, Nexplanon, 3-Oxodesogestrel, 3-keto-Desogestrel

Catalog No.S4673 Purity: 99.90%

Etonogestrel (Implanon, Nexplanon, 3-Oxodesogestrel, 3-keto-Desogestrel) is a synthetic form of the naturally occurring female sex hormone progesterone. It binds to the cytoplasmic progesterone receptors in the reproductive system and subsequently activates progesterone receptor mediated gene expression.

Etonogestrel Chemical Structure

CAS: 54048-10-1

Selleck's Etonogestrel has been cited by 2 publications

Purity & Quality Control

Batch: Purity: 99.90%

99.90

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Biological Activity

Description	Etonogestrel (Implanon, Nexplanon, 3-Oxodesogestrel, 3-keto-Desogestrel) is a synthetic form of the naturally occurring female sex hormone progesterone. It binds to the cytoplasmic progesterone receptors in the reproductive system and subsequently activates progesterone receptor mediated gene expression.

In vitro
In vitro	Etonogestrel induces FKBP51 mRNA and protein expression in cultured HESCs^[1].
Cell Research	Cell lines	Human endometrial stromal cells (HESCs)
	Concentrations	100 nM
	Incubation Time	7 days
	Method	Thawed HESCs are grown to confluence in basal medium (BM), a phenol red-free 1:1 v/v mix of Dulbecco's MEM/Ham's F-12, with 100U/ml penicillin, 100 μg/ml streptomycin, 0.25 μg/ml fungizone complex supplemented with 10% charcoal-stripped calf serum. Confluent HESCs are incubated in parallel in BM with 0.1% ethanol (vehicle control) or 10nM E2(estradiol) or E2+100 nM P4(progesterone), or 100 nM of ETO(etonogestrel) or 100 nM of MPA(medroxyprogesterone acetate). After 7 days, the cultures are washed twice with 1X phosphate-buffered saline (PBS) to remove residual serum, then placed in a serum-free defined media (DM) comprising BM plus ITS+ (insulin, transferrin, selenious and linoleic acid) premix, 5 μM of FeSO4, 50 μM of ZnSO4, 1 nM of CuSO4, 20 nM of Na2SeO3 trace elements, 50 μg/ml of ascorbic acid and 50 ng/ml of recombinant epidermal growth factor for 24h. The cultures are washed twice with 1X PBS to remove the residual steroids and DM containing corresponding vehicle control or steroids is added to the cultures for 6h or 24h. At the end of incubation periods, HESCs are washed with ice-cold 1X PBS and stored at −70°C until used for total RNA and protein extraction.

In Vivo
In vivo	Etonogestrel (Implanon, Nexplanon, 3-Oxodesogestrel, 3-keto-Desogestrel) is a synthetic form of the naturally occurring female sex hormone progesterone.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT04882137	Withdrawn	Contraceptive Usage	Nemours Children''s Clinic\|Merck Sharp & Dohme LLC	September 1 2023	Not Applicable
NCT05360550	Completed	Pregnancy Prevention	Lupin Research Inc	July 5 2022	Phase 2
NCT05040282	Completed	Contraception	Assiut University	October 1 2021	Not Applicable
NCT05002738	Completed	Contraception	University of Colorado Denver	September 10 2021	Phase 4

References

Chemical lnformation & Solubility

Molecular Weight	324.46	Formula	C₂₂H₂₈O₂
CAS No.	54048-10-1	SDF	Download Etonogestrel SDF
Smiles	CCC12CC(=C)C3C(C1CCC2(C#C)O)CCC4=CC(=O)CCC34
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 65 mg/mL ( (200.33 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 65 mg/mL

Water : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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% DMSO + % + % Tween 80 + % ddH₂O

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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