Home Immunology & Inflammation ADC Cytotoxin inhibitor - Immunology & Inflammation related inhibitor Isopsoralen For research use only.

Isopsoralen

Synonyms: angelicin

Isopsoralen (Angelicin), also known as angelicin, is a constituent of roots and leaves of angelica with anti-inflammatory activity and regulates LPS-induced inflammation via inhibiting MAPK/NF-κB pathways. It also shows antiviral activity against gammaherpesviruses.

Isopsoralen Chemical Structure

Isopsoralen Chemical Structure

CAS: 523-50-2

Purity & Quality Control

Batch: Purity: 99.91%
99.91

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Biological Activity

Description Isopsoralen (Angelicin), also known as angelicin, is a constituent of roots and leaves of angelica with anti-inflammatory activity and regulates LPS-induced inflammation via inhibiting MAPK/NF-κB pathways. It also shows antiviral activity against gammaherpesviruses.
In vitro
In vitro Isopsoralen treatment stimulates the accumulation of cartilage nodules in a dose-dependent manner. Isopsoralen enhances the expressions of chondrogenic marker genes such as collagen II, collagen X, OCN, Smad4 and Sox9 in a time-dependent manner. Furthermore, isopsoralen induces the activation of extracellular signal-regulated kinase (ERK) and p38 MAP kinase, but not that of c-jun N-terminal kinase (JNK). Isopsoralen significantly enhances the protein expression of BMP-2 in a time-dependent manner and mediates a chondromodulating effect by BMP-2 or MAPK signaling pathways[2].
Cell Research Cell lines ATDC5 cells
Concentrations 0-1 μM
Incubation Time 24 h
Method

ATDC5 cells are seeded in 96-well plate at a density of 5×103 cells/well and incubated overnight in media supplemented with 10% FBS followed by treatment with various concentrations of isopsoralen. After 24h of incubation, cells are washed with phosphate-buffered saline (PBS) and then treated with media containing 100 μg/ml MTT for 2 h at 37℃. The cells are then washed with PBS, and dissolved in 200 μl of DMSO. The resulting solubilized purple formazan is quantified by measuring the absorbance at a wavelength of 540 nm using a spectrophotometer.
In Vivo
In vivo Isopsoralen has significant osteoprotective effect in female and male mice with sex hormone deprivation. After administration of isopsoralen for 8 weeks, Bone strength increases and trabecular bone microstructure improves[1]. After intravenous administration to wistar rats, the elimination half-lives of isopsoralen is 5.35 h. The area under the curves of the tissues for isopsoralen decrease in the following order: kidney > lung > liver > heart > spleen > brain. After oral administration to Wistar rats, the elimination half-lives of isopsoralen is 5.56 h, and its bioavailability is 70.35%[3].
Animal Research Animal Models ICR mice
Dosages 10 mg/kg and 20 mg/kg
Administration intragastrically

Chemical lnformation & Solubility

Molecular Weight 186.16 Formula

C11H6O3
CAS No. 523-50-2 SDF Download Isopsoralen SDF
Smiles C1=CC2=C(C=CO2)C3=C1C=CC(=O)O3
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 14 mg/mL ( (75.2 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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