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Medroxyprogesterone

Name: Medroxyprogesterone
Brand: Selleck Chemicals
SKU: S3635

Synonyms: MP

Catalog No.S3635 Purity: 99.48%

Medroxyprogesterone (MP) is a synthetic pregnane steroid and a derivative of progesterone. It is a potent progesterone receptor agonist.

Medroxyprogesterone Chemical Structure

CAS: 520-85-4

Purity & Quality Control

Batch: Purity: 99.48%

99.48

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Biological Activity

Description	Medroxyprogesterone (MP) is a synthetic pregnane steroid and a derivative of progesterone. It is a potent progesterone receptor agonist.

In vitro
In vitro	Medroxyprogesterone acetate (MPA, 10 nM) treatment for 48 h induces proliferation of the cells (1.6-fold induction). MPA induces cyclin D1 expression (3.3-fold induction). MPA increases both the protein level (2.2-fold induction) and promoter activity (2.7-fold induction) of cyclin D1 in MCF-7 cells transfected with PRB but not with PRA. Although MPA transcriptionally activates cyclin D1 expression, cyclin D1 promoter does not have progesterone-responsive element-related sequence. MPA induces the transient phosphorylation of Akt (2.7-fold induction at 5 min) and also phosphorylation of inhibitor of NFkappaBalpha (IkappaBalpha) (2.3-fold induction)^[1]. MPA activates glucocorticoid receptors, which could mimic part of the anti-atherosclerotic effects of glucocorticoids, and has, on the other hand, also anti-androgenic activity, which might diminish protective oestrogen effects^[2].
Cell Research	Cell lines	T47D human breast cancer cells
	Concentrations	10 nM
	Incubation Time	4 days
	Method	The cells are plated at a density of 10 or 40 × 10⁴ cells/well in 12-well plates and allowed to attach overnight. The cells are then growth arrested by incubation in phenol red-free DMEM with 10% charcoal-stripped serum for 24 h followed by treatment with vehicle or MPA by exchanging the culture medium containing these agents with fresh medium every 24 h for 4 d. A Neubauer chamber is used to count cells, and trypan blue experiments are carried out in quadruplicate.

In Vivo
In vivo	Long-term treatment with MPA and MPA + E2 increases arterial thrombosis despite inhibitory effects of MPA on atherosclerosis in ApoE-deficient mice. Increased thrombin formation, reduced smooth muscle content and remodelling of non-collagenous plaque matrix may be involved in the pro-thrombotic effects. Thus, MPA exhibits differential effects on arterial thrombosis and on atherosclerosis. In monkeys, MPA interferes with anti-atherosclerotic oestrogen effects whereas progesterone does not^[2].

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT06193135	Not yet recruiting	Infertility Female\|Reproductive Sterility	Instituto Valenciano de Infertilidad IVI VALENCIA	March 1 2024	Not Applicable
NCT05188222	Not yet recruiting	Left Ventricular Dysfunction\|Quality of Recovery\|Right Ventricular Dysfunction	Jewish General Hospital	February 1 2024	Phase 4
NCT05452928	Not yet recruiting	Herpes Simplex 2\|Meningitis Viral	Jacob Bodilsen\|Aalborg University Hospital	January 1 2024	Phase 4

References

Chemical lnformation & Solubility

Molecular Weight	344.49	Formula	C₂₂H₃₂O₃
CAS No.	520-85-4	SDF	Download Medroxyprogesterone SDF
Smiles	CC1CC2C(CCC3(C2CCC3(C(=O)C)O)C)C4(C1=CC(=O)CC4)C
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 68 mg/mL ( (197.39 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 14 mg/mL

Water : Insoluble

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In vivo
Batch:

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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