Choose Your Country or Region

Nortriptyline hydrochloride

Name: Nortriptyline hydrochloride
Brand: Selleck Chemicals
SKU: S3698
Rating: 5 (1 reviews)

Synonyms: Desitriptyline HCl, ELF-101 hydrochloride, EN-7048 hydrochloride, Desmethylamitriptyline hydrochloride

Catalog No.S3698 Purity: 99.97%

Nortriptyline hydrochloride (Desitriptyline, ELF-101, EN-7048, Desmethylamitriptyline) is the hydrochloride salt form of nortriptyline, a tricyclic antidepressant agent used for short-term treatment of various forms of depression.

Nortriptyline hydrochloride Chemical Structure

CAS: 894-71-3

Selleck's Nortriptyline hydrochloride has been cited by 1 publication

Purity & Quality Control

Batch: S369801 DMSO] 59 mg/mL] false] Water] 59 mg/mL] false] Ethanol] 59 mg/mL] false Purity: 99.97%

99.97

Related compound libraries

Choose Selective Autophagy Inhibitors

Biological Activity

Description	Nortriptyline hydrochloride (Desitriptyline, ELF-101, EN-7048, Desmethylamitriptyline) is the hydrochloride salt form of nortriptyline, a tricyclic antidepressant agent used for short-term treatment of various forms of depression.

In vitro
In vitro	Nortriptyline inhibits the vascular Kv channels in a concentration-dependent and state-independent manner independently of serotonin reuptake inhibition^[1]. It is a tricyclic antidepressant. At the cellular level, nortriptyline is inhibitory to many proteins and responses such as astroglial inwardly rectifying Kir4.1 channels, human neutrophil phagocytosis and oxidative burst, human cytochrome P-450 enzymes, opioid receptors, Ca2+-activated K+ channels, and priming of human neutrophils. Nortriptyline induces a significant [Ca2+]i rise and decreases viability in PC3 cells, MG63 human osteosarcoma, and renal tubular cells^[2]. Nortriptyline also exhibits anticancer activity in several different types of cells. For example,in human cutaneous melanoma cells,NTP has a half maximal inhibitory concentration(IC50) of 9 μM compared with 27 μM and 33 μM for clomipramine and amitriptyline, respectively. Nortriptyline induces cell cycle arrest and apoptosis in TCCSUP and MBT-2 cells^[3].
Cell Research	Cell lines	PC3 cells
	Concentrations	50-500 µM
	Incubation Time	24 h
	Method	Measurement of cell viability is based on the ability of cells to cleave tetrazolium salts by dehydrogenases. Augmentation in the amount of developed color directly correlates with the number of live cells. Cells are seeded in 96-well plates at 10,000 cells/well in culture medium for 24 h in the presence of several concentrations of nortriptyline. The cell viability-detecting reagent WST-1 is added to samples after nortriptyline treatment, and cells are incubated for 30 min in a humidified atmosphere.

In Vivo
In vivo	Nortriptyline inhibits tumor growth in mice inoculated with MBT-2 cells. Low concentrations of NTP (0.1-5 μM) confer neuroprotective effects, downregulate cytosolic phospholipase A2, and prevent mitochondrial depolarization with minimal toxicity in both astrocytes and mice. In contrast, higher concentrations of NTP (10-50 μM) have anti-tumor effects on human osteosarcoma and cutaneous melanoma cells^[3].
Animal Research	Animal Models	male C3H/HeN mice
	Dosages	10 or 20 mg/kg
	Administration	i.p.

References

Chemical lnformation & Solubility

Molecular Weight	299.84	Formula	C₁₉H₂₁N.HCl
CAS No.	894-71-3	SDF	Download Nortriptyline hydrochloride SDF
Smiles	CNCCC=C1C2=CC=CC=C2CCC3=CC=CC=C31.Cl
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 59 mg/mL ( (196.77 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 59 mg/mL

Ethanol : 59 mg/mL

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):