Home DNA Damage/DNA Repair DNA/RNA Synthesis inhibitor Cyclophosphamide For research use only.

Cyclophosphamide

Synonyms: NSC 26271,Endoxan, Cytoxan, Neosar, Procytox, Revimmune, Cytophosphane

Cyclophosphamide(NSC 26271,Endoxan, Cytoxan, Neosar, Procytox, Revimmune, Cytophosphane) is a nitrogen mustard alkylating agent from the oxazophorines group.

Cyclophosphamide Chemical Structure

Cyclophosphamide Chemical Structure

CAS: 50-18-0

Selleck's Cyclophosphamide has been cited by 8 Publications

1 Customer Review

Purity & Quality Control

Batch: Purity: 100%
100

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Biological Activity

Description Cyclophosphamide(NSC 26271,Endoxan, Cytoxan, Neosar, Procytox, Revimmune, Cytophosphane) is a nitrogen mustard alkylating agent from the oxazophorines group.
Targets
DNA replication [1]
In Vivo
In vivo

Cyclophosphamide is an alkylating agent with cytotoxic and immunosuppressive activities. Cyclophosphamide requires enzymatic and chemical activation to release active phosphoramide mustard which produces the interstrand and intrastrand DNA crosslinks at guanine N-7 positions. Cyclophosphamide exerts its cytotoxic activity by inhibiting nuclear DNA replication. The mechanism of Cyclophosphamide -induced immunosuppression is possibly due to direct cytotoxic effect of the drug on immunocompetent lymphocytes, particulafly those that have undergone antigenic differentiation and division. Cyclophosphamide has its greatest effect on B cells with relative T cell-sparing effect. [1] Trials in various tumor-bearing animals confirmed this in vivo activity and demonstrated fairly potent antitumor effect. Cyclophosphamide, in combination with other antineoplastic agents, is used for the treatment of various cancers, including breast, lymphoid and pediatric malignancies. [2]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06186700 Not yet recruiting Breast Cancer Female Mansoura University December 25 2023 Phase 2
NCT06085742 Recruiting Breast Cancer University of Illinois at Chicago October 2023 Phase 2
NCT05800041 Not yet recruiting Gout Tophus RenJi Hospital|Westlake Therapeutics April 10 2023 Early Phase 1

Chemical lnformation & Solubility

Molecular Weight 261.09 Formula

C7H15Cl2N2O2P
CAS No. 50-18-0 SDF Download Cyclophosphamide SDF
Smiles ClCCN(CCCl)[P]1(=O)NCCCO1
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 52 mg/mL ( (199.16 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 52 mg/mL

Water : 43 mg/mL


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In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
Why S1217 Cyclophosphamide shows no activity in vitro assays?

Answer:
The activity of this product in vitro is controversial and has not been fully determined. Cyclophosphamide is a prodrug and may need Cytochrome P450 to convert it to the active form: 4-hydroxy cyclophosphamide. It is widely used in vivo, if you are going to use it in vitro, you may need to supplement Cytochrome P450 exogenously.

DNA/RNA Synthesis Signaling Pathway Map

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