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Clodronate Disodium

Name: Clodronate Disodium
Brand: Selleck Chemicals
SKU: S4108
Rating: 5 (2 reviews)

Catalog No.S4108 Purity: 99%

Clodronate Disodium, a bisphosphonate, is a potent antiosteolytic agent which inhibits bone resorption.

Clodronate Disodium Chemical Structure

CAS: 22560-50-5

Selleck's Clodronate Disodium has been cited by 2 publications

Purity & Quality Control

Content >99%
Finest quality approved by Nature Medicine
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Biological Activity

Description	Clodronate Disodium, a bisphosphonate, is a potent antiosteolytic agent which inhibits bone resorption.

In vitro
In vitro	Clodronate significantly decreases total viability of cultures of J774 cells with EC50 of 300 μM, while liposome-encapsulated Clodronate decreases total viability of cultures of J774 cells with EC50 of 1 μM. Clodronate and liposome-encapsulated Clodronate is metabolized to a nonhydrolyzable adenosine triphosphate (ATP) analog, adenosine 5'-(beta, gamma-dichloromethylene) triphosphate, which can be detected in J774 cell extracts by using fast protein liquid chromatography. ^[1] Clodronate induces apoptosis in isolated osteoclasts. Clodronate, when administered in liposomes, also induces apoptosis in rat peritoneal macrophages in vitro and in liver macrophages of mice in vivo but not in murine macrophage-like RAW-264 cells. ^[2] Clodronate delivered into macrophages by liposome will kill these cells as a result of intracellular accumulation and irreversible metabolic damage. ^[3] Clodronate encapsulated in liposomes (clodrolip) efficiently depletes the phagocytic cells in the murine F9 teratocarcinoma and human A673 rhabdomyosarcoma mouse tumour models resulting in significant inhibition of tumour growth ranging from 75 to >92%, depending on therapy and schedule. ^[4] Clodronate, a bisphosphonate that lacks a nitrogen, does not inhibit protein isoprenylation but can be metabolized intracellularly to a beta-gamma-methylene (AppCp-type) analog of ATP, which is cytotoxic to macrophages in vitro. Clodronate is metabolited to AppCCl(2)p, and AppCCl(2)p inhibits mitochondrial oxygen consumption by a mechanism that involves competitive inhibition of the ADP/ATP translocase. ^[5]

References

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Chemical lnformation & Solubility

Molecular Weight	288.86	Formula	CH₄Cl₂O₆P₀.2Na
CAS No.	22560-50-5	SDF	Download Clodronate Disodium SDF
Smiles	C(P(=O)(O)[O-])(P(=O)(O)[O-])(Cl)Cl.[Na+].[Na+]
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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